Homework Answers
Ans)7) b. antisense
Explaination:
Antisense having a sequence of nucleotides complementary to (and hence capable of binding to) a coding (or sense) sequence, which may be either that of the strand of a DNA double helix which undergoes transcription, or that of a messenger RNA molecule.
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or d. transposons
refers to short DNA or RNA sequences, termed oligonucleotides, which are designed to...
1 Which of the following represents one of the mechanisms by which antisense agents exert their...
1 Which of the following represents one of the mechanisms by which antisense agents exert their effect? a. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that promotes translation to the corresponding protein b. Antisense oligonucleotides prevent the natural silencing of targeted mRNA sequences C. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that cleaves the mRNA d. Antisense oligonucleotides inhibit all types of gene splicing 2 The...
“Unlike what happens in DNA replication, where both strands are copied, only one of the two...
“Unlike what happens in DNA replication, where both strands are copied, only one of the two strands is transcribed into mRNA. The DNA strand that contains the gene is sometimes called the sense strand, or coding strand, and the DNA strand that gets transcribed to give RNA is called the antisense strand, or noncoding strand. Because the sense strand and the antisense strand are complementary, and because the DNA antisense strand and the newly formed RNA strand are also complementary,...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in the top (5' to 3) strand. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG- 3' 3-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC- 5' Once Cas9 binds to a PAM sequence, it unwinds the DNA. If the guide RNA matches the DNA sequence next to the PAM, the guide RNA will bind to the complementary DNA strand. If not, the DNA will zip back together and Cas9 will keep binding to other PAM sequences until it finds the...
Which of these is involved in the transcription of tRNA genes? A. RNA Pol 1 B....
Which of these is involved in the transcription of tRNA genes? A. RNA Pol 1 B. RNA Pol 2 C.RNA Pol 3 D.RNA Pol 4 E. Not sure A promoter is.... A. Proteins that defines the beginning of a gene B. DNA elements that help define the beginning of a gene C. It is the DNA sequence at the start of a gene D. Not sure Promoters TATA Start site What is the TATA box? A. It's a DNA sequence...
The DNA primers used in PCR are a. complementary to DNA sequences at both ends of...
The DNA primers used in PCR are a. complementary to DNA sequences at both ends of the DNA sequence of interest. b. attached to the gene of interest by ligase. c. produced when a gene of interest is read by restriction enzymes. d. identical to the entire base sequence of one strand of the DNA.
Transcription is terminated, when a characteristic RNA structure _____ is formed by local RNA palindromic sequences...
Transcription is terminated, when a characteristic RNA structure _____ is formed by local RNA palindromic sequences at the 3’- end. The structure interacts with RNAP-bound NusA, causing dissociation of RNAP from template DNA. The RNAP dissociation is stimulated by sequence-specific binding of _____ proteins to the transcribed RNA, enhancing termination of transcription. Select one: a. hairpin ;;;; rho b. hairpin ;;;; sigma c. beta-turn ;;;; rho d. beta-turn ;;;; sigma e. None of these
3. Below are several DNA sequences that are mutated compared with the wild-type sequence: 3’ -...
3. Below are several DNA sequences that are mutated compared with the wild-type sequence: 3’ - T A C T G A C T G A C G A T C - 5’. Each is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the template strand. a. Construct the complementary DNA sequences (indicating 5’ and 3’ ends) for each mutated DNA sequence. b. Transcribe (indicating 5’ and 3’ ends) the...
34. Transcription is terminated, when a characteristic RNA structure is formed by local RNA palindromic sequences...
34. Transcription is terminated, when a characteristic RNA structure is formed by local RNA palindromic sequences at the 3-end. The structure interacts with RNAP-bound NusA, causing dissociation of RNAP from template DNA. The RNAP dissociation is stimulated by sequence specific binding of proteins to the transcribed RNA, enhancing termination of transcription Select one a. hairpin - rho b. hairpin sigma c. beta-turn rho d. beta-turn sigma e. None of these
10. Select all TRUE answers for the following terms. (7 points) DNA Replication (2 points) a....
10. Select all TRUE answers for the following terms. (7 points) DNA Replication (2 points) a. Copies both strands of DNA at the same time. b. Occurs only during S phase of cell cycle. C. Generates RNA from DNA template. d. Necessary for mitosis, but not meiosis. DNA Transcription (2 points) a. Substitutes Guanine (DNA) for Uracil (RNA). b. Produces RNA with complementary sequence to DNA template. C. Generates RNA from both strands of DNA at the same time. d....
The general term Choose... A hybridization B Southern blotting C PCR D annealing refers to the...
The general term Choose... A hybridization B Southern blotting C PCR D annealing refers to the process in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes are formed between molecules with complementary sequences.
1 Which of the following represents one of the mechanisms by which antisense agents exert their effect? a. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that promotes translation to the corresponding protein b. Antisense oligonucleotides prevent the natural silencing of targeted mRNA sequences C. A short DNA sequence binds to specific regions of complementary mRNA, inducing a nuclease that cleaves the mRNA d. Antisense oligonucleotides inhibit all types of gene splicing 2 The...
3. The partial gene (DNA) sequence below contains multiple PAM sequences. Highlight six PAM sequences in the top (5' to 3) strand. 5'-GCACGGCGGAGCGGTTCTTGGCAGCGGCCGCACGATCTCGTTGCCGCCGG- 3' 3-CGTGCCGCCTCGCCAAGAACCGTCGCCGGCGTGCTAGAGCAACGGCGGCC- 5' Once Cas9 binds to a PAM sequence, it unwinds the DNA. If the guide RNA matches the DNA sequence next to the PAM, the guide RNA will bind to the complementary DNA strand. If not, the DNA will zip back together and Cas9 will keep binding to other PAM sequences until it finds the...
Which of these is involved in the transcription of tRNA genes? A. RNA Pol 1 B. RNA Pol 2 C.RNA Pol 3 D.RNA Pol 4 E. Not sure A promoter is.... A. Proteins that defines the beginning of a gene B. DNA elements that help define the beginning of a gene C. It is the DNA sequence at the start of a gene D. Not sure Promoters TATA Start site What is the TATA box? A. It's a DNA sequence...
34. Transcription is terminated, when a characteristic RNA structure is formed by local RNA palindromic sequences at the 3-end. The structure interacts with RNAP-bound NusA, causing dissociation of RNAP from template DNA. The RNAP dissociation is stimulated by sequence specific binding of proteins to the transcribed RNA, enhancing termination of transcription Select one a. hairpin - rho b. hairpin sigma c. beta-turn rho d. beta-turn sigma e. None of these
10. Select all TRUE answers for the following terms. (7 points) DNA Replication (2 points) a. Copies both strands of DNA at the same time. b. Occurs only during S phase of cell cycle. C. Generates RNA from DNA template. d. Necessary for mitosis, but not meiosis. DNA Transcription (2 points) a. Substitutes Guanine (DNA) for Uracil (RNA). b. Produces RNA with complementary sequence to DNA template. C. Generates RNA from both strands of DNA at the same time. d....
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