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3 Result Table 2 Oxygen Consumption During Germination 4 6 Vial 1 Vial 3 Vial 2 7 8 Distance Pipet reading Pipet reading moved (ml)reading moved (ml) Distance Pipet Distance moved 9 (ml) 10 11 O ml 0 ml 12 0 ml 0.86ml 0.97ml 0.97ml min. reading 13 14 15 min. reading 0.77 ml 0.09 ml 0.9 ml 0.07 ml 0.88 ml 0.09 ml 16 17 10 min reading 0.11 ml 0.7 ml 0.16 ml 0.86 ml 0.82 ml 0.15 ml 18 19 20 15 min. 0.65 ml 0.21 ml 0.83 ml 0.14 ml 0.78ml 0.19 ml 21 reading 22 23 20 min. 0.56 ml 0.76 ml 0.21 ml 0.3ml 0.78ml 0.19 ml 24 reading 25 26 27 Correction 0.19 ml 0.19 ml 28 29 orrected Net Change 0.11 ml 30 0.02 ml 31 32 te of oxygen 33 onsumption 0.0055 ml 0.001ml ml/min 34 Trendline 35 Rate of 36 -=----=---- Which seed variety had the highest O2 consumption and which had the lowest? How does the rate of 02 production differ when calculated using just the endpoint data (20 minutes) verses plotting a trendline? Why? Why did the water move in the pipet?

Answer 1

Oxygen Consumption during Germination 1 0.9 E0.8 S 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 20 1 10 15 Time (min) Vial 1 Vial 2 Vial 3 Linear (Vial 3) Linear (Vial 1) . Linear (Vial 2) - Oxygen Consumption (ml) un

1. According to the trend lines of the three vials, the net O₂ consumption is highest for Vial 1 and lowest for Vial 2

2. (Question error - its CO₂ production not O₂ production)
If we use just the endpoint data, the CO₂ production comes out to be higher than if we use the trend line data because, using the trend line we can figure the accurate representation of carbon dioxide produced since during germination, the gas exchange during respiration is small and gradually increases as the biomass of the seed grows. This accurate representation is not seen when calculating using just the endpoint data.

3. The water moved in the pipette because the germinating seeds consumed the oxygen present and released equal amounts of carbon dioxide. The carbon dioxide reacted with KOH present in the vial to form Potassium carbonate. This removed carbon dioxide from the environment and the resultant pressure inside the vial decreased which pulled in water from the pipette.