Homework Answers
The % of Transformation Efficiency = Practical yield / Theorical yield * 100.
The practical yield = 10^6/ ug and
the theoritical yield = 10^8/ ug.
So, The TE = 10^6/10^8 * 100 = 1.
so, the answer is 1.
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4. You have prepared a batch of competent E coli cells and found its transformation efficiency...
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2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
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1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
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of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
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