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4. You plan to separate a mixture of the two compounds below using a column chromatography...
1) In a column chromatography procedure, if two compounds were eluted in the same fraction, how...
1) In a column chromatography procedure, if two compounds were eluted in the same fraction, how would you separate them? 2) why is it important to not let the column dry out?
Chromatography I Questions Name CHE 201 Date The following solvent systems were found to separate compounds...
Chromatography I Questions Name CHE 201 Date The following solvent systems were found to separate compounds A and B by column chromatography: • A: hexane/ethyl acetate 10:1 • B: hexane/ethyl acetate 10:5 Which is the more polar compound, A or B? Explain your answer for credit, 2) In order to separate a mixture of A and B as in problem (1) by column chromatography: a. Which solvent system must be used first? Why? b. Which compound will elute from the...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
6a) During experiment 7, you performed column chromatography in order to separate two main compounds from...
6a) During experiment 7, you performed column chromatography in order to separate two main compounds from tomato paste. What are these two compounds? 6b) Is this separation technique a chemical or physical method of separation? 6c) What color is each of these compounds? What about their molecular structure gives rise to their color? 6d) During the experiment, we only observed one band on the column even though we should have observed two. However, you may have noticed, we had in...
Page 4. A stadent wanted to separate compounds A, B and C by column chromatography. Before...
Page 4. A stadent wanted to separate compounds A, B and C by column chromatography. Before deciding on a mobile phase, he ran TLC in the three solvent systems he was considering for this experiment, solvent 1, solvent 2 and solvent 2 (shown below).(10 points) Based on the TLC results what can you conclude reganding the polarity of compounds A, B and CT a. b. What can you conclude reganding the polarity of the mobille phases solvent 1, 2 and...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
These questions are from an experiment where we had a mixture of Ferrocene, acetylferrocene and diacetyl...
These questions are from an experiment where we had a mixture of Ferrocene, acetylferrocene and diacetyl ferrocene and we separated each by using column chromatography. Before doing the column chromatography we did TLC analysis to ensure that we did, in fact, have a mixture of these 3 compounds. During the column chromatography we first eluted the ferrocene using pure hexane. Then we eluted the acetylferrocene using a 1:1 mixture of hexane and methylene chloride. Then we eluted the diacetylferrocene using...
QUESTION 7 18 erystas? Cold methandl QUESTION 8 A crude extract of ginger contains compounds X, Y...
QUESTION 7 18 erystas? Cold methandl QUESTION 8 A crude extract of ginger contains compounds X, Y and Z (structures shown above) In an attempt to separate these compounds using column chromatography with Silca Gel as the stationary phase and methanol as the mobile phase. predict the sequence of elution (starting from the first to be eluted) that Talya would expect Z First O x-Finst Z Last O z- First Y Last OY First X Middle X-Middle Save All A...
4. Your research adviser hands you a 3-component mixture and tells you to separate them using...
4. Your research adviser hands you a 3-component mixture and tells you to separate them using column chromatography. You already have some lab savvy so you know to test first by tlc to see if you can find a good solvent system for your column. Shown below are your first 2 developed plates and the solvent systems used. Obviously neither system is appropriate, but you now have a good idea of what to try next (you really, really understand this...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase?...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
Chromatography I Questions Name CHE 201 Date The following solvent systems were found to separate compounds A and B by column chromatography: • A: hexane/ethyl acetate 10:1 • B: hexane/ethyl acetate 10:5 Which is the more polar compound, A or B? Explain your answer for credit, 2) In order to separate a mixture of A and B as in problem (1) by column chromatography: a. Which solvent system must be used first? Why? b. Which compound will elute from the...
2. An unknown compound was analyzed using thin layer chromatography, its constituted of two components (A and B) as shown in the TLC (Silica coated stationary phase; Chloroform and Ethyl acetate as mobile phase). The mixture was then loaded onto column with silica as the stationary phase and the same solvent system was used. (2 points) Column Chromatography TLC time A В Predict the order of elution of the compounds (A and B). which one would be eluted first/last and...
6a) During experiment 7, you performed column chromatography in order to separate two main compounds from tomato paste. What are these two compounds? 6b) Is this separation technique a chemical or physical method of separation? 6c) What color is each of these compounds? What about their molecular structure gives rise to their color? 6d) During the experiment, we only observed one band on the column even though we should have observed two. However, you may have noticed, we had in...
Page 4. A stadent wanted to separate compounds A, B and C by column chromatography. Before deciding on a mobile phase, he ran TLC in the three solvent systems he was considering for this experiment, solvent 1, solvent 2 and solvent 2 (shown below).(10 points) Based on the TLC results what can you conclude reganding the polarity of compounds A, B and CT a. b. What can you conclude reganding the polarity of the mobille phases solvent 1, 2 and...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
QUESTION 7 18 erystas? Cold methandl QUESTION 8 A crude extract of ginger contains compounds X, Y and Z (structures shown above) In an attempt to separate these compounds using column chromatography with Silca Gel as the stationary phase and methanol as the mobile phase. predict the sequence of elution (starting from the first to be eluted) that Talya would expect Z First O x-Finst Z Last O z- First Y Last OY First X Middle X-Middle Save All A...
4. Your research adviser hands you a 3-component mixture and tells you to separate them using column chromatography. You already have some lab savvy so you know to test first by tlc to see if you can find a good solvent system for your column. Shown below are your first 2 developed plates and the solvent systems used. Obviously neither system is appropriate, but you now have a good idea of what to try next (you really, really understand this...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
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