What are the limitations of Transient Transfection technique? What can’t it show you?

Question

Question

Answer 1

Answer #1

In transient transfection technique ,added nucleicacid's effect is temporary means not integrated in genome of host cell.

It's limitations are-

Expression is not stable means express only for some time upto it is not degraded by host machinery.

That nucleic acid can't be passes from one generation to next generation so that it don't require to be added again and again.

Expression of this gene is also affected by environment factors.

It can't show long term effect of that gene on host cell

Answer 2

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