As we know that is In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter.The genes are transcribed together into an mRNA strand and either translated together in the cytoplasm, or undergo splicing to create monocistronic mRNAs that are translated separately, i.e. several strands of mRNA that each encode a single gene product .so it is The lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. ans it also work in the body
Lactase is a protein, an enzyme that is produced in the small intestine. The human body uses lactase to break down lactose into galactose and glucose, which is then absorbed into the bloodstream. If lactase levels are low, the lactose does not break down and it does not absorb into the bloodstream.
Regulation of the Lactose SystemThe salient features of transcriptional regulation in prokaryotes can be seen in the regulation of expression of the enzymes necessary for lactose metabolism in the bacterium Escherichia coli
The lac Structural Genes
The metabolism of lactose requires two enzymes: a permease to transport lactose into the cell and β-galactosidase to cleave the lactose molecule to yield glucose and galactose
Catabolite Repression of the lac Operon: Choosing the Best Sugar to Metabolize
An additional control system is superimposed on the repressor–operator system. This control system is thought to have evolved because the cell can capture more energy from the breakdown of glucose than it can from the breakdown of other sugars. If both lactose and glucose are present, the synthesis of β-galactosidase is not induced until all the glucose has been utilized.
Draw and describe the regulation of the Escherichia colilac operon in the following situations: 1. In the absence of lactose (disregard presence or absence of glucose). 2. In the presence of lactose (disregard presence or absence of glucose). 3. In the absence of glucose (disregard presence or absence of lactose). Finally, describe where in the gene expression process this regulation is taking place.
In the space below draw the lactose operon in an environment that LACKS lactose. On your image label the following items: DNA f. Lac I gene Lac promoter g. Lac operator Lac Z gene h. RNA polymerase Lac Y gene i. mRNA message (if made) e. Lac repressor protein
4. Briefly draw and/or describe the functions of the genes contained in the lac operon. Why is a low level of lac operon expression necessary for it to respond to the presence of lactose ?
a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. PROMOTER OPERATOR Lactose Enzyme 1 Lsctose Enzyme2 Lactose Enzyme 3 NO b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES 2. Bobby Joe is fasting today, how will the E. coli in her stomach respond to the lack of Tryptophan? a) For the trp operon, will the repressor...
5. Briefly describe positive control in the lac operon when glucose is absent and lactose is present in high amounts.
The lac operon codes for enzymes that break down lactose, but this operon is only used when needed. This includes: 1. When the repressor is bound at the operator 2.When RNA polymerase is bound to the promotor WITHOUT the CAP molecule 3.When the CAP and cAMP molecules are bound to the RNA polymerase and lactose is bound to the repressor 4.When the CAP and cAMP molecules are bound to the RNA polymerase and lactose is NOT bound to the repressor
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon? CAMP is a repressor of the lactose operon and turns off gene expression of the lactose operon CAMP is an activator and prevents the repressor protein from binding to the operator region of the operon which allows for the lactose operon to be turned "on". 5 CAMP is an inducer of the lactose operon and also an activator since it prevents the repressor protein...
9. Using the diagram of the lac operon (regulates transcription of genes for lactose metabolizing enzymes) below, draw the appropriate positions for RNA polymerase and the CAP activator molecule when CAMP is present. Then draw the appropriate position for the repressor molecule when lactose is present as an inducer. Finally, indicate whether or not transcription occurs. (8 pts) Promoter Operator Gene 1 Gene 2 Gene 3
In E coli the lac operon will express enzymes for the digestion of (Glucose/Lactose) when it is present, but if both the sugar mentioned before and (Glucose/ Lactose) are present the lac operon is repressed, resulting in the (Glucose/ Lactose) being used first. If there are high levels of cAMP, this means (Glucose/Lactose) levels are low, this results in a (Represser/Promoter) being activated and (Decreasing/Increasing) transcription of the lac operon.
7. How does a repressor protein turn off the lac operon? 8. How does lactose turn on the lac operon? 9. Complete the table to describe the role of each regulatory region or molecule in the operation of the lac operon.