Question

Part A -Why is cDNA used in RNAseq? Because cDNA is more stable than RNA Because it captures all the genes expressed in a tissue or a cell Existing sequencing protocols are designed for sequencing DNA All the above are true Submit Request Answer Part B-Why do scientists measure gene expression? Because Gene expression is static, and the same gene is always expressed the same Because Gene expression realtes to changes in the genomic DNA Because Gene expression is dynamic, and the same gene may act in different ways under different circumstances. None of the above. Gene expression is obsolete based on the the new massive parallel sequencing technologies Submit Request Answer

Answer 1

Part A, the 3 are true because

the DNA is a more stable molecule than RNA .RNA is more labile to action of Rnasas and temperature

the technique of sequencing has been designed for using DNA as template . For analysing expression of particular genes you have to use the information that is copy in a RNA messenger molecule.cDNA is a copy of the information of RNA m and is obtained through a reverse transcription using RNA messenger as template

B- the correct is the third sentence. Gene expression is studied because changes at these level can influence dramatically different biological processes depending of the different circumstances under which the genes are expressed