Homework Answers
| Organism | Chloramphenicol | Ciprofloxacin | Trimethoprim | Penicillin | ||||
| Zone of diameter | S/R | Zone of diameter | S/R | Zone of diameter | S/R | Zone of diameter | S/R | |
| E.coli | 30mm | S | 24mm | S | 7mm | R | 0mm | R |
| S.aureus | 27mm | S | 24mm | S | 5mm | R | 30mm | S |
Chloramphenicol is the antibiotic to which E.coli is more susceptible. Because, zone of inhibtion diameter is 30mm for chloramphenicol.
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Q4) Let assume you have done the experiment on the lab and the zones of inhibition...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of these antibiotics would be classified as broad-spectrum antibiotics? lics? (will kill both 5a. Ampicillin is a form of penicillin. If the zone of inhibition for a susceptible strain of E. coli is mm, and for a strain of S. aureus is 29 mm, which one is inhibited by the lower concentration or ampicillin? 5b. Would you need a higher dose of ampicillin to cure...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
An antibiotic sensitivity test was performed for S. aureas, B. cereus, E coli and S. typhimurim....
An antibiotic sensitivity test was performed for S. aureas, B.
cereus, E coli and S. typhimurim. The antibiotics used are
Gentamycin (GM), Chloramphenicol (C), Streptomycin (S), Ampicillin
(AM), Tetracycline (TE) and Erythromycin (E). The diameters of the
zones of inhibition were measured for each antibiotic and recorded.
S = susceptible. R = resistant. I = intermediate. Do the results
align with the expected results for each organism?
Lab Report 6 Antimicrobial Activity Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity...
Lab
Report 6 Antimicrobial Activity
Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity Test Chemotherapeutic Gram negative Gram Positive agent E. coli S. aureus B. cereus Zone Susceptibility Zone Susceptibility Zone Susceptibility Size Size Size Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Chemotherapeutic agent Spectrum of Activity (Broad or limited) Type(s) of Microorganisms (Gram positive or negative) Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Part B. Synergistic effect of drug combinations Culture and drug combination Appearance of Zone Synergistic...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure 1. Record the zone size and the susceptibility of each test organism to the chemotherapeutic agent as resistant (R), intermediate (1), or sensitive (S) in the charts below. E coli P. vulgaris GRAM-NEGATIVE Paeruginosa Zone Size Susceptibility ACID-FAST M. smegmatis Zone Size Susceptibility Chemotherapeutic Zone Size Zone Size Agent Susceptibility Susceptibility Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Sulfanilamide 32 32 GRAM-POSITIVE E. faecalis S....
E.coli P. aeruginosa S. aureus The diameter for the Zone of Inhibition for Gentomycin is 25...
E.coli P. aeruginosa S. aureus The diameter for the Zone of Inhibition for Gentomycin is 25 mm. What does this represent? O E. coli is sensitive to GM E. coli never has antibiotic resistance. OGM has no effect on E. coli The numbers must be checked with manufacturer's tables E. coli is resistant to GM
Using the data from the table on page 23, identify one narrow spectrum and one broad...
Using the data from the table on page 23, identify one narrow spectrum and one broad spectrum antibiotic. Give an example to explain why you chose each antibiotic. Narrow spectrum: Broad spectrum: Evaluation of the NON-ANTIBIOTIC products. Product Zone (mm) Name of organism tested by your group: BiophyicOCCUS aurous Antibiotic Zone (mm) Antibiotic Zone (mm Ampicillin mm m mipene Imipenem 30 mm Azithromycin Kanamycin 20mm Cefazolin Neomycin 20mm Chloramphenicolm Penicillin 20mm Ciprofloxacin 5 mm Polymyxin amm Colistina Streptomycin 2 mm...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
can you please help me with Qs 1-6 thanks ! LAB EXERCISE #6: Kirby-Bauer Disk Diffusion...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
Serratia marcescens MH 2 S - 20 16.7 mm mm Wuna Antibiotic 1. Ampicillin, 10 ug...
Serratia marcescens MH 2 S - 20 16.7 mm mm Wuna Antibiotic 1. Ampicillin, 10 ug 2. Tetracycline, 30 ug 3. Chloramphenicol, 30 ug 4.Vancomycin, 30 ug 25 mm mm Nassau After reviewing the Lab Module 6 - Kirby-Bauer Antibiotic Susceptibility Testing Introduction and Background document and the supplemental lab videos, complete the lab report below. Use the reference chart on the susceptibilities of antibiotics on page 2. Indicate for each strain below based on measurements of the diameter of...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of these antibiotics would be classified as broad-spectrum antibiotics? lics? (will kill both 5a. Ampicillin is a form of penicillin. If the zone of inhibition for a susceptible strain of E. coli is mm, and for a strain of S. aureus is 29 mm, which one is inhibited by the lower concentration or ampicillin? 5b. Would you need a higher dose of ampicillin to cure...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
An antibiotic sensitivity test was performed for S. aureas, B.
cereus, E coli and S. typhimurim. The antibiotics used are
Gentamycin (GM), Chloramphenicol (C), Streptomycin (S), Ampicillin
(AM), Tetracycline (TE) and Erythromycin (E). The diameters of the
zones of inhibition were measured for each antibiotic and recorded.
S = susceptible. R = resistant. I = intermediate. Do the results
align with the expected results for each organism?
Lab
Report 6 Antimicrobial Activity
Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity Test Chemotherapeutic Gram negative Gram Positive agent E. coli S. aureus B. cereus Zone Susceptibility Zone Susceptibility Zone Susceptibility Size Size Size Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Chemotherapeutic agent Spectrum of Activity (Broad or limited) Type(s) of Microorganisms (Gram positive or negative) Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Part B. Synergistic effect of drug combinations Culture and drug combination Appearance of Zone Synergistic...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure 1. Record the zone size and the susceptibility of each test organism to the chemotherapeutic agent as resistant (R), intermediate (1), or sensitive (S) in the charts below. E coli P. vulgaris GRAM-NEGATIVE Paeruginosa Zone Size Susceptibility ACID-FAST M. smegmatis Zone Size Susceptibility Chemotherapeutic Zone Size Zone Size Agent Susceptibility Susceptibility Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Sulfanilamide 32 32 GRAM-POSITIVE E. faecalis S....
E.coli P. aeruginosa S. aureus The diameter for the Zone of Inhibition for Gentomycin is 25 mm. What does this represent? O E. coli is sensitive to GM E. coli never has antibiotic resistance. OGM has no effect on E. coli The numbers must be checked with manufacturer's tables E. coli is resistant to GM
Using the data from the table on page 23, identify one narrow spectrum and one broad spectrum antibiotic. Give an example to explain why you chose each antibiotic. Narrow spectrum: Broad spectrum: Evaluation of the NON-ANTIBIOTIC products. Product Zone (mm) Name of organism tested by your group: BiophyicOCCUS aurous Antibiotic Zone (mm) Antibiotic Zone (mm Ampicillin mm m mipene Imipenem 30 mm Azithromycin Kanamycin 20mm Cefazolin Neomycin 20mm Chloramphenicolm Penicillin 20mm Ciprofloxacin 5 mm Polymyxin amm Colistina Streptomycin 2 mm...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
Serratia marcescens MH 2 S - 20 16.7 mm mm Wuna Antibiotic 1. Ampicillin, 10 ug 2. Tetracycline, 30 ug 3. Chloramphenicol, 30 ug 4.Vancomycin, 30 ug 25 mm mm Nassau After reviewing the Lab Module 6 - Kirby-Bauer Antibiotic Susceptibility Testing Introduction and Background document and the supplemental lab videos, complete the lab report below. Use the reference chart on the susceptibilities of antibiotics on page 2. Indicate for each strain below based on measurements of the diameter of...
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