A nutrient agar plate labeled 10^-5mL produced 154 colonies after incubation. a.What was the cell density...
A nutrient agar plate labeled 10^-5mL produced 154 colonies after incubation.
a.What was the cell density in the original sample?
b. What combination(s) of volumes and dilution factors could have been used to inoculate this plate? (note two possibilities)
Homework Answers
a) Original cell density = CFU / (dilution x Volume)
= 154 / 10 -5 x 0.1 mL
= 154 x 106
= 1.54 x 108 CFU/ml
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