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Your protein of interest will not form crystals, and it is 100 kDa in size. You...

Your protein of interest will not form crystals, and it is 100 kDa in size. You would still like to study it structurally. What technique will you explore next, and why? What limitations might you encounter?

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Answer #1

The following image is of my notes and will guide you to techniques used for the analysis of structure of protein

9:48 PM 0.86K/s 4G 37% Titre Several methods are currently used to determine the structure of a protein, including X-ray crystallography, NMR spectroscopy, and electron microscopy. Each method has advantages and disadvantages. In each of these methods, the scientist uses many pieces of information to create the final atomic model. Primarily, the scientist has some kind of experimental data about the structure of the molecule. For X-ray crystallography, this is the X-ray diffraction pattern. For NMR spectroscopy, it is information on the local conformation and distance between atoms that are close to one another. In electron microscopy, it is an image of the overall shape of the molecule.

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