Homework Answers
Add Answer to:
6. If you have two proteins with unknown concentrations (4 points): -sample A: a protein that...
Question 6 Which of the following statements regarding a protein with 4 domains must be true?...
Question 6 Which of the following statements regarding a protein with 4 domains must be true? A protein with 4 domains must have 4 different kinds of secondary structures. O A protein with 4 domains must be composed of 4 polypeptide chains. A protein with 4 domains must have 4 regions that fold independently. A protein with 4 domains must have a quaternary structure. Question 7 Histone proteins are typically... o rich in disulfide bonds O composed of a random...
32. Which of the following would make a protein of a large size move faster through...
32. Which of the following would make a protein of a large size move faster through an SDS gel electrophoresis separation? a. add more protein to your sample b. add more buffer c. remove some buffer d. decrease the acrylamide concentration of the gel e. increase the acrylamide concentration of the gel 33. Which of the following statements are accurate? i. SDS binds to proteins, introducing a negative charge which is proportional to the length of the protein primary structure....
9. In the mixture of proteins you have protein A: 35 kDa, protein B: 15 kDa,...
9. In the mixture of proteins you have protein A: 35 kDa, protein B: 15 kDa, protein C: 58 kDa, protein D: 150 kDa. Propose the chromatographical method to separate them. Write in which order they would elute from the column. 10. What is the best chromatographical method to separate fluorescein and amide black? They are organic dyes.
PDX-1 must bind to DNA in order to function. A particular arrangement of amino acids with...
PDX-1 must bind to DNA in order to function. A particular arrangement of amino acids with polar r-groups determines the sequence of nitrogenous bases the PDX-1 can bind to via hydrogen bonds; however, a separate binding pocket of the protein helps it attach to the DNA backbone. What would you predict the characteristics of those amino acids are? Positively Charged Polar Uncharged Negatively Charged Nonpolar
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting,...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...
The gene encoding protein Bog in E. coli has been changed by a point mutation in...
The gene encoding protein Bog in E. coli has been changed by a point mutation in its open reading frame. Originally the gene encoded a 108 amino acid long functioning protein whose amino acid composition favored nonpolar and negatively charged amino acids. After the mutation it encodes a 110 amino acid long non-functioning protein that is now rich in polar uncharged amino acids and positively charged amino acids. Which mutation scenario best explains this observation. A) A frame-shift caused by...
Sequence Comparisons Proteins called molecular chaperones (described in Chapter 4) assist in the process of protein...
Sequence Comparisons Proteins called molecular chaperones (described in Chapter 4) assist in the process of protein folding. One class of chaperone found in organisms from bacteria to mammals is heat shock protein 90 (Hsp90). All Hsp90 chaperones contain a 10 amino acid "signature sequence," which allows for ready identification of these proteins in sequence databases. Two representations of this signature sequence are shown below. (a) In this sequence, which amino acid residues are invariant (conserved across all species)? (b) At...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity chromatography but also to ___________. A. overexpress the proteins in bacteria B. to reduce toxicity of the foreign proteins C. for proper folding and keeping the proteins soluble D. to trace the cytological location of the foreign proteins 15. You have constructed an insulin-GST fusion protein and expressed it in E. coli. You want to separate the recombinant...
need only part a and C. please and thank you 6. Suppose you have a mixture...
need only part a and C. please and thank you
6. Suppose you have a mixture of five proteins listed in the table below: Protein pl Mol. Welght, kDa Homer 4.6 69 Liza 10.7 13 Marge Maggie Bart 5 40 6.4 8.5 7 27 Indicate the order in which the proteins elute from a Bio-Gel p-10 gel-filtration column (starting with the on that elutes first). Is this the best resin to separate all the components of this mixture? Why? If...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
Question 6 Which of the following statements regarding a protein with 4 domains must be true? A protein with 4 domains must have 4 different kinds of secondary structures. O A protein with 4 domains must be composed of 4 polypeptide chains. A protein with 4 domains must have 4 regions that fold independently. A protein with 4 domains must have a quaternary structure. Question 7 Histone proteins are typically... o rich in disulfide bonds O composed of a random...
32. Which of the following would make a protein of a large size move faster through an SDS gel electrophoresis separation? a. add more protein to your sample b. add more buffer c. remove some buffer d. decrease the acrylamide concentration of the gel e. increase the acrylamide concentration of the gel 33. Which of the following statements are accurate? i. SDS binds to proteins, introducing a negative charge which is proportional to the length of the protein primary structure....
9. In the mixture of proteins you have protein A: 35 kDa, protein B: 15 kDa, protein C: 58 kDa, protein D: 150 kDa. Propose the chromatographical method to separate them. Write in which order they would elute from the column. 10. What is the best chromatographical method to separate fluorescein and amide black? They are organic dyes.
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...
Sequence Comparisons Proteins called molecular chaperones (described in Chapter 4) assist in the process of protein folding. One class of chaperone found in organisms from bacteria to mammals is heat shock protein 90 (Hsp90). All Hsp90 chaperones contain a 10 amino acid "signature sequence," which allows for ready identification of these proteins in sequence databases. Two representations of this signature sequence are shown below. (a) In this sequence, which amino acid residues are invariant (conserved across all species)? (b) At...
need only part a and C. please and thank you
6. Suppose you have a mixture of five proteins listed in the table below: Protein pl Mol. Welght, kDa Homer 4.6 69 Liza 10.7 13 Marge Maggie Bart 5 40 6.4 8.5 7 27 Indicate the order in which the proteins elute from a Bio-Gel p-10 gel-filtration column (starting with the on that elutes first). Is this the best resin to separate all the components of this mixture? Why? If...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
Most questions answered within 3 hours.
-
Free content. Free software. Freemium pricing models. Viral
popularity. Disappearing gatekeepers. These are the disrupters of...
asked 37 minutes ago -
Consider the simple linear regression model: HARD1 =
β0 + β1*SCORE + є, where
є ~...
asked 1 hour ago -
In the Chesapeake region during the mid-17th
century how similar was the experience of an independent...
asked 3 hours ago -
. A 2.50 M stock solution was used to prepare a dilute NaOH
solution with a...
asked 4 hours ago -
Write a program that reads in a text file, infile.txt, and
prints out all the lines...
asked 6 hours ago -
Which of the following orbits have a total of zero energy (based
on the common convention)?...
asked 7 hours ago -
Find the expected count and the contribution to the chi-square
statistic for the (Group 1, No)...
asked 8 hours ago -
Suppose you are purchasing a meal and with every meal a you
receive one of 10...
asked 8 hours ago -
A 75.0 kg 0erson is riding in a car moving at 20.0 m/s when the
car...
asked 8 hours ago -
What does Δp = QR mean in words?
Pushing a viscous fluid through a pipe requires...
asked 8 hours ago -
We know that system.out.println (“object number” + n ); is
legal.String name; int n; name =...
asked 8 hours ago -
Come up with a real-life problem (open-ended) and solve it by
designing and writing a Java...
asked 8 hours ago