Question

9. In the mixture of proteins you have protein A: 35 kDa, protein B: 15 kDa, protein C: 58 kDa, protein D: 150 kDa. Propose the chromatographical method to separate them. Write in which order they would elute from the column. 10. What is the best chromatographical method to separate fluorescein and amide black? They are organic dyes.

Answer 1

9 solu hon 1. The exchange chromatographic method may be used to separate the protein mixture of the above said molecular masers. It is a type of chromatography that sepalely. The components of the mixture based on chargy. 9 4 column of changed saltionary pased on solid support called an ion exchange resis. a The method involves two steps in the first step the charged molecules of the loaded sample get bound to the column resin =) In this second step. the proces of eluhon is made to carry gradient is used out by. A salt to separate proteins. TE a buffer containing more than one protein used with anion exchange resin. then the most negance charged protein will be most attacked to the will therefore, elute and stahonary phase and will therefore, elul the protein with the highest positive charge elute first