Question

You have a mixture of three proteins: Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z, MW 75 kDa, pl 5.0 2) You have a cation exchange (acidic) column, low salt (100 mM NaCl) and high salt (1 M NaCI) buffer at pH 7.0. If you loaded the above mixture of proteins on the column in low salt buffer, which proteins would most likely be retained on the column? If you loaded the above mixture of proteins on the column in high salt buffer, which proteins would most likely be retained on the column?

Answer 1

Cation ex hamge coleumm Meliks on the attration hettu pen ety charyed otationary phare analy and the vely charged he erely charyedfd teual 6aund tny he ant por t meodium imnert of hange fchethages rngte Immobi anin e the unface thange oattinay stum kch he met chrge the Pt aut frot sin the protin eey thaed PHY PI pPZ thn whm, LA tP of puten fatily N anu PH 7 at awill e beund he chned f aro enp of catin the exyhang u t than PH va 6 7 Gin A buith co lu changed t doe, met 8 S proten 42