Question

a) Suppose you label dividing cells with ^32P. In which DNA molecules will the radioactivity be incorporated if you were to look during metaphase? b) Suppose you are examining the above cells after replication. In which DNA molecules will the radioactivity be present? In one picture, draw a schematic indicating how DNA is synthesized bi-directionally. The schematic should indicate which are the lagging strands, which are the leading strands, where the RNA primers are, and the 3' and 5' orientation of the strands. Indicate the Okazaki fragments in the figure. b) Why does the lagging strand have Okazaki fragments while the leading strand does not? Suppose you had a bacterial mutant that was defective for topoisomerase, one that was defective for topoisomerase, one that was defective for primase, and one that was defective for DNA polymerase. Your student mis-labelled the three cultures. How could you differentiate among the three mutants? Describe two ways in which prokaryotic and eukaryotic replication differs.

Answer 1

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