1. What is the approach of targeted gene knockout? And what are the advantages and disadvantages of using this technique?
1. What is the approach of targeted gene knockout? And what are the advantages and disadvantages...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
What is Montessori Approach? What are the advantages and disadvantages of sending a child to a Montessori school?
2. Gene knockout in mouse A. Outline the sequence of steps necessary to generate a gene knockout in the mouse. Be sure to include the information you need to start the procedure. B. The targeted insertion of your transgene in embryonic stem (ES) cells has a very low probability of success (roughly 1 in 10,000 cells tested). Based on what you know about molecular biology, why is the probability of success so low for this step? What feature of the...
Identify the advantages and disadvantages of using the few suppliers approach.
1 what are the advantages and disadvantages of each hostile take over approach a tender offer, proxy fight, and a creeping tender offer? 2 what examples of hostile takeovers have been in the news this past year? 3. What approach was used in these takeovers?
What were the advantages and disadvantages of taking a “business union” approach as opposed to advocating a labor political party?
Describe the advantages and disadvantages for an organization using a research-based approach to problem solving.
Explain the Matrix approach to manage projects, and its advantages and disadvantages
What are the advantages and disadvantages of outdoor advertising? What are the advantages and disadvantages of tv advertising? What are the advantages and disadvantages of magazine advertising? 75 word minimum