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2) List the major targets of an antimicrobial drug. What do all of these targets have...
2) List the major targets of an antimicrobial drug What do all of these ta common?...
2) List the major targets of an antimicrobial drug What do all of these ta common? all of these targets have in 3) Why is the Kirby-Bauer test standardized? 4) What is the advantage of the broth dilution tests for antibiotic sensitivity over Kirby Bauer? 5) In general, why are Gram negative bacteria resistant to some of the antibiotics that Gram positive bacteria are sensitive to? 6) Fill in the following chart. ANTIBIOTIC CELLULAR TARGET AMPICILLIN CHLORAMPHENICOL ERYTHROMYCIN GENTAMICIN PENICILLIN...
Lab Report 6 Antimicrobial Activity Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity...
Lab
Report 6 Antimicrobial Activity
Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity Test Chemotherapeutic Gram negative Gram Positive agent E. coli S. aureus B. cereus Zone Susceptibility Zone Susceptibility Zone Susceptibility Size Size Size Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Chemotherapeutic agent Spectrum of Activity (Broad or limited) Type(s) of Microorganisms (Gram positive or negative) Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Part B. Synergistic effect of drug combinations Culture and drug combination Appearance of Zone Synergistic...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure 1. Record the zone size and the susceptibility of each test organism to the chemotherapeutic agent as resistant (R), intermediate (1), or sensitive (S) in the charts below. E coli P. vulgaris GRAM-NEGATIVE Paeruginosa Zone Size Susceptibility ACID-FAST M. smegmatis Zone Size Susceptibility Chemotherapeutic Zone Size Zone Size Agent Susceptibility Susceptibility Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Sulfanilamide 32 32 GRAM-POSITIVE E. faecalis S....
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of...
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of these antibiotics would be classified as broad-spectrum antibiotics? lics? (will kill both 5a. Ampicillin is a form of penicillin. If the zone of inhibition for a susceptible strain of E. coli is mm, and for a strain of S. aureus is 29 mm, which one is inhibited by the lower concentration or ampicillin? 5b. Would you need a higher dose of ampicillin to cure...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
Gram +ve Resistant Strains (%) 100 BOS Ribosome 90 50 Ribosome 80 Folate Pathway 70 DNA/RNA...
Gram +ve Resistant Strains (%) 100 BOS Ribosome 90 50 Ribosome 80 Folate Pathway 70 DNA/RNA Synthesis 60 Cell Wall Synthesis Fell Membrane Synercid Antibiotic (20 kg/ml) Apramycin Gentamicin Neomycin Streptomycin Tetracycline Minocycline Tigecycline Clindamycin Chloramphenicol Gram -ve 100 305 Ribosome 90 505 Ribosome Folate Pathway 70 DNA/RNA Synthesis Cell Wall Synthesis Resistant Strains (%) Antibiotic (20 wg/ml) Erythromycin Telithromycin Linezolid Sulfamethoxazole Apramycin Trimethoprim Gentamicin Ciprofloxacin Neomycin Novobiocin Streptomycin Rifampicin Tetracycline Vancomycin Meropenem Minocycline Cefotaxime Tigecycline Cephalexin Clindamycin Fosfomycin Chloramphenicol...
Using the data from the table on page 23, identify one narrow spectrum and one broad...
Using the data from the table on page 23, identify one narrow spectrum and one broad spectrum antibiotic. Give an example to explain why you chose each antibiotic. Narrow spectrum: Broad spectrum: Evaluation of the NON-ANTIBIOTIC products. Product Zone (mm) Name of organism tested by your group: BiophyicOCCUS aurous Antibiotic Zone (mm) Antibiotic Zone (mm Ampicillin mm m mipene Imipenem 30 mm Azithromycin Kanamycin 20mm Cefazolin Neomycin 20mm Chloramphenicolm Penicillin 20mm Ciprofloxacin 5 mm Polymyxin amm Colistina Streptomycin 2 mm...
Question 5 1.5 points Save Answer An organism identified as Staphylococcus was challenged by several antibiotics...
Question 5 1.5 points Save Answer An organism identified as Staphylococcus was challenged by several antibiotics using the Kirby-Bauer protocol. EACH of the following drugs showed a zone of inhibition of 19 mm. Given these results, which antibiotic would be most useful in treatment against this organism? (Use the information on the reference chart below.) Drug Resistant Intermediate Susceptible Ampicillin < 28 mm 28 mm > 28 mm Erythromycin < 13 mm 13-22 mm > 22 mm Penicillin G 28...
1. Of the 4 labeled disinfectants, which have antimicrobial activity and which do not? 2. Of...
1. Of the 4 labeled disinfectants, which have antimicrobial activity and which do not? 2. Of those that have antimicrobial activity, which one has the greatest? 9. 3. What is present in the plate that indicates antimicrobial activity? 4. Could there be any reason other than "significant antimicrobial activity that a zone of inhibition is very large? shutterstock.com . 1461769682 SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 1. What does each disc have a precise amount of? 2....
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
2) List the major targets of an antimicrobial drug What do all of these ta common? all of these targets have in 3) Why is the Kirby-Bauer test standardized? 4) What is the advantage of the broth dilution tests for antibiotic sensitivity over Kirby Bauer? 5) In general, why are Gram negative bacteria resistant to some of the antibiotics that Gram positive bacteria are sensitive to? 6) Fill in the following chart. ANTIBIOTIC CELLULAR TARGET AMPICILLIN CHLORAMPHENICOL ERYTHROMYCIN GENTAMICIN PENICILLIN...
Lab
Report 6 Antimicrobial Activity
Lab report 6 Antimicrobial Activity Observations Part A. Kirby-Bauer Antibiotic Sensitivity Test Chemotherapeutic Gram negative Gram Positive agent E. coli S. aureus B. cereus Zone Susceptibility Zone Susceptibility Zone Susceptibility Size Size Size Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Chemotherapeutic agent Spectrum of Activity (Broad or limited) Type(s) of Microorganisms (Gram positive or negative) Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Part B. Synergistic effect of drug combinations Culture and drug combination Appearance of Zone Synergistic...
16 Name: Section Lab Report Date Observations and Results Part A: Kirby-Bauer Antibiotic Sensitivity Test Procedure 1. Record the zone size and the susceptibility of each test organism to the chemotherapeutic agent as resistant (R), intermediate (1), or sensitive (S) in the charts below. E coli P. vulgaris GRAM-NEGATIVE Paeruginosa Zone Size Susceptibility ACID-FAST M. smegmatis Zone Size Susceptibility Chemotherapeutic Zone Size Zone Size Agent Susceptibility Susceptibility Penicillin Streptomycin Tetracycline Chloramphenicol Gentamicin Vancomycin Sulfanilamide 32 32 GRAM-POSITIVE E. faecalis S....
3. Which of these antibiotics would be classified as narrow-spectrum antibiotics? grampos sneg) 4. Which of these antibiotics would be classified as broad-spectrum antibiotics? lics? (will kill both 5a. Ampicillin is a form of penicillin. If the zone of inhibition for a susceptible strain of E. coli is mm, and for a strain of S. aureus is 29 mm, which one is inhibited by the lower concentration or ampicillin? 5b. Would you need a higher dose of ampicillin to cure...
Kirby-Bauer Test time-lapse with E. coli and S. aureus Elapsed Time: 24 hours coll Il 0 :18/1:11 Ampicillin Gentamicin Neomycin Vancomycin Tetracycline Bacitracin Trimethoprim/ Sulfamethoxazole Optochin Penicillin Erythromycin Streptomycin Amoxicillin/ Clavulanic Acid E. coli HD Q : II 0 - 0:33/1:11 3. Which of your organisms appears more susceptible to penicillin? 4. Which of your organisms appears difficult to treat with the selected antibiotics? 5. What is the difference between G+ and G-in reference to the selected antibiotics? 6. Which...
Gram +ve Resistant Strains (%) 100 BOS Ribosome 90 50 Ribosome 80 Folate Pathway 70 DNA/RNA Synthesis 60 Cell Wall Synthesis Fell Membrane Synercid Antibiotic (20 kg/ml) Apramycin Gentamicin Neomycin Streptomycin Tetracycline Minocycline Tigecycline Clindamycin Chloramphenicol Gram -ve 100 305 Ribosome 90 505 Ribosome Folate Pathway 70 DNA/RNA Synthesis Cell Wall Synthesis Resistant Strains (%) Antibiotic (20 wg/ml) Erythromycin Telithromycin Linezolid Sulfamethoxazole Apramycin Trimethoprim Gentamicin Ciprofloxacin Neomycin Novobiocin Streptomycin Rifampicin Tetracycline Vancomycin Meropenem Minocycline Cefotaxime Tigecycline Cephalexin Clindamycin Fosfomycin Chloramphenicol...
Using the data from the table on page 23, identify one narrow spectrum and one broad spectrum antibiotic. Give an example to explain why you chose each antibiotic. Narrow spectrum: Broad spectrum: Evaluation of the NON-ANTIBIOTIC products. Product Zone (mm) Name of organism tested by your group: BiophyicOCCUS aurous Antibiotic Zone (mm) Antibiotic Zone (mm Ampicillin mm m mipene Imipenem 30 mm Azithromycin Kanamycin 20mm Cefazolin Neomycin 20mm Chloramphenicolm Penicillin 20mm Ciprofloxacin 5 mm Polymyxin amm Colistina Streptomycin 2 mm...
Question 5 1.5 points Save Answer An organism identified as Staphylococcus was challenged by several antibiotics using the Kirby-Bauer protocol. EACH of the following drugs showed a zone of inhibition of 19 mm. Given these results, which antibiotic would be most useful in treatment against this organism? (Use the information on the reference chart below.) Drug Resistant Intermediate Susceptible Ampicillin < 28 mm 28 mm > 28 mm Erythromycin < 13 mm 13-22 mm > 22 mm Penicillin G 28...
1. Of the 4 labeled disinfectants, which have antimicrobial activity and which do not? 2. Of those that have antimicrobial activity, which one has the greatest? 9. 3. What is present in the plate that indicates antimicrobial activity? 4. Could there be any reason other than "significant antimicrobial activity that a zone of inhibition is very large? shutterstock.com . 1461769682 SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 1. What does each disc have a precise amount of? 2....
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
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