Question

A 3.75 mL unknown sample containing red and blue dyes was separated on a solid phase extraction column. As it was eluted from the column, each sample was diluted to a total volume of 50.00 mL in a volumetric flask with the appropriate solvent. UV spectrophotometry was used to determine the amount of dye in the diluted sample. The data collected by the students in the lab are provided below. Sample R40 standard oncentration 0.025 M Absorbance Path Length 0.323@ 505 nm .00 cm olution 1 standard solution 0.15 M Diluted R40 sample Unknown Diluted B1 sample Unknown 6916 at 630 nm 425 @505 nm 3415 00 cm 00 cm 630 nm .00 cm Using the data provided above, determine the concentration of red dye in the original undiluted sample. Enter your answer in the following format: X.X4 (the last digit is given to avoid rounding errors). Do NOT include units. You must put leading zeroes. s Previous

The last digit in the answer is four X.X4

Answer 1

The concentration of red dye in the original undiluted sample = 0.44 M