EXPERIMENT #13 - Partitioning and Spectroscopic Identification of Food Dyes Answer the following questions 1. In...
EXPERIMENT #13 - Partitioning and Spectroscopic Identification of Food Dyes
Answer the following questions
1. In the column, the stationary phase is hydrophilic
hydrophobic
| Tries 0/1 |
2. Which dye will be removed first from the column? (with 15%
ethanol)
red
blue
| Tries 0/1 |
3. Which dye will be removed last from the column? (with 95%
ethanol)
blue
yellow
| Tries 0/1 |
4. If a standard dye solution, with concentration of 10 μg/mL has an absorbance of 0.720 at a wavelength of 630 nm and the unknown sample has an absorbance of 0.430 at the same wavelength, what is the concentration of the unknown sample, in μg/mL
μg/mL
Homework Answers
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EXPERIMENT #13 - Partitioning and Spectroscopic
Identification of Food Dyes
Answer the following questions
1. In...
The last digit in the answer is four X.X4 A 3.75 mL unknown sample containing red...
The last digit in the answer is four X.X4
A 3.75 mL unknown sample containing red and blue dyes was separated on a solid phase extraction column. As it was eluted from the column, each sample was diluted to a total volume of 50.00 mL in a volumetric flask with the appropriate solvent. UV spectrophotometry was used to determine the amount of dye in the diluted sample. The data collected by the students in the lab are provided below. Sample...
PROCEDURE A. ABSORBANCE SPECTRA 1. Obtain three cuvettes. Add-2mL of the following samples to them: red...
PROCEDURE A. ABSORBANCE SPECTRA 1. Obtain three cuvettes. Add-2mL of the following samples to them: red dye, blue dye, yellow dye. 2. Obtain a spectrum for each dye according to the provided directions (water is the blank). 3. For each of the three dyes (red, yellow, and blue) record the wavelength for the largest peak in the visible range (380 nm to 750 nm), Record this in the data table below. Save the red dye for part B. 4. Identify...
Need help for questions 2 and 4 G. C. 1-7 Determination of the Dye Concentration in...
Need help for questions 2 and 4
G. C. 1-7 Determination of the Dye Concentration in a Powdered Drink G. C. 1-7: PART 2 GROUP DISCUSSION QUESTIONS Lab partners' name: 1. Your TA will provide you with the correct concentration of the dye in the powdered drink solution. Calculate the percentage error for your data. (experimental value - accepted value) accepted value 2. The empirical formula for the Brilliant Blue dye is CanHyN,O,S, Na2 and the formula weight i 79286...
1. Draw the boundaries for the va below: es for the various ranges of colors of...
1. Draw the boundaries for the va below: es for the various ranges of colors of visible of colors of visible light on the waveleriyle 750 nm 400 nm 700 nm 500 nm 600 nm 2. Below is an absorbance spectrum for a violet/purple the color scale you drew in Question 1, wh dye? Which color(s) is/are most transmitted? plet/purple dye called crystal violet. According to or(s) is/are most absorbed by the violet Absorbance 600 100 500 Wavelength, nm 3....
1. Draw the boundaries for the various ta below: bus ranges of colors of visible light on the wa le light on the w...
1. Draw the boundaries for the various ta below: bus ranges of colors of visible light on the wa le light on the wavelelige 750 nm 400 nm 700 nm 500 nm 600 nm 2. Below is an absorbance spectrum for a violet/purple the color scale you drew in Question 1, wn dye? Which color(s) is/are most transmitted? olet/purple dye called crystal violet. According to Ich color(s) is/are most absorbed by the violet Absorbance 600 300 400 500 Wavelength, nm...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
Percent by mass of dye in Fruit Loops cereal 11.56*100 QUESTIONS: 1. If the molar extinction coefficient (E) of a...
Percent by mass of dye in Fruit Loops cereal 11.56*100 QUESTIONS: 1. If the molar extinction coefficient (E) of a compound is 2.5 x 108 moli'cmat $28 nm and the Abs Cat 528 nm) 0.254 Dind the concentration of that solution. (assume a path length of 1.0 cm) 2. If a student measures a sample that gives an Abs = 1.85 at the 2max, is this data reliable? If not, then what might they do to improve their data? 3....
Please type your answer so I can read it, and I just need help for question...
Please type your answer so I can read it, and I just
need help for question one ( Hypothesis ) don't worry about the
rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as...
need hep with these two questions! here is the information for the lab K2Cr₂O + H₂...
need hep with these two questions!
here is the information for the lab
K2Cr₂O + H₂ C₂04 2H2O sklor (C204) [H202₂/2 H2otca 2k12 2 cr=x2 (+8ly kzcrgo7+ I 142 C204-21420 2 k cellow). (1) 2 - 2 H2O toc -=S7 30-4521 2. Calculate the theoretical yield of K[Cr(C,0.),(H,0), 2H,0. 3. What is the theoretical percentage by weight of Cr in K[Cr(C,0.),(H,0), 2H,0? using the spectrophotometer at your desk, at the wavelength of maximum absorbance of the blue-green chromium ion, measure...
The last digit in the answer is four X.X4
A 3.75 mL unknown sample containing red and blue dyes was separated on a solid phase extraction column. As it was eluted from the column, each sample was diluted to a total volume of 50.00 mL in a volumetric flask with the appropriate solvent. UV spectrophotometry was used to determine the amount of dye in the diluted sample. The data collected by the students in the lab are provided below. Sample...
PROCEDURE A. ABSORBANCE SPECTRA 1. Obtain three cuvettes. Add-2mL of the following samples to them: red dye, blue dye, yellow dye. 2. Obtain a spectrum for each dye according to the provided directions (water is the blank). 3. For each of the three dyes (red, yellow, and blue) record the wavelength for the largest peak in the visible range (380 nm to 750 nm), Record this in the data table below. Save the red dye for part B. 4. Identify...
Need help for questions 2 and 4
G. C. 1-7 Determination of the Dye Concentration in a Powdered Drink G. C. 1-7: PART 2 GROUP DISCUSSION QUESTIONS Lab partners' name: 1. Your TA will provide you with the correct concentration of the dye in the powdered drink solution. Calculate the percentage error for your data. (experimental value - accepted value) accepted value 2. The empirical formula for the Brilliant Blue dye is CanHyN,O,S, Na2 and the formula weight i 79286...
1. Draw the boundaries for the va below: es for the various ranges of colors of visible of colors of visible light on the waveleriyle 750 nm 400 nm 700 nm 500 nm 600 nm 2. Below is an absorbance spectrum for a violet/purple the color scale you drew in Question 1, wh dye? Which color(s) is/are most transmitted? plet/purple dye called crystal violet. According to or(s) is/are most absorbed by the violet Absorbance 600 100 500 Wavelength, nm 3....
1. Draw the boundaries for the various ta below: bus ranges of colors of visible light on the wa le light on the wavelelige 750 nm 400 nm 700 nm 500 nm 600 nm 2. Below is an absorbance spectrum for a violet/purple the color scale you drew in Question 1, wn dye? Which color(s) is/are most transmitted? olet/purple dye called crystal violet. According to Ich color(s) is/are most absorbed by the violet Absorbance 600 300 400 500 Wavelength, nm...
Percent by mass of dye in Fruit Loops cereal 11.56*100 QUESTIONS: 1. If the molar extinction coefficient (E) of a compound is 2.5 x 108 moli'cmat $28 nm and the Abs Cat 528 nm) 0.254 Dind the concentration of that solution. (assume a path length of 1.0 cm) 2. If a student measures a sample that gives an Abs = 1.85 at the 2max, is this data reliable? If not, then what might they do to improve their data? 3....
Please type your answer so I can read it, and I just
need help for question one ( Hypothesis ) don't worry about the
rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as...
need hep with these two questions!
here is the information for the lab
K2Cr₂O + H₂ C₂04 2H2O sklor (C204) [H202₂/2 H2otca 2k12 2 cr=x2 (+8ly kzcrgo7+ I 142 C204-21420 2 k cellow). (1) 2 - 2 H2O toc -=S7 30-4521 2. Calculate the theoretical yield of K[Cr(C,0.),(H,0), 2H,0. 3. What is the theoretical percentage by weight of Cr in K[Cr(C,0.),(H,0), 2H,0? using the spectrophotometer at your desk, at the wavelength of maximum absorbance of the blue-green chromium ion, measure...
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