Question

4.) Southen Blots You run a Southern Blot using genomic DNA and a probe aligning to the piece of DNA below. ECoRI EcoRI BamHl BamH 2000- 1600 200 0 200 400 600 800 000 1200 1400 1600 1800 2000bp BamH EcoRI BamHI Probe EcoRI BamH Draw the bands you expect to see if the genomic DNA is cut to completion with 400- EcoRI, BamHI, or both (EcoRI+BamHI) before size separation. (8 points)

Answer 1

1)0------------------1000 1kb BamH1

2)0--------------------------1800 1.8 kb BamH1 (probe attached)

3) 0--------400 0.4 BamH1 and EcoR1

4) 0------------------------1600 1.6 BamH1 and EcoR1(probe)

5) 400----------1000 0.6 EcoR1 and Bam H1

6) 400------------------1600 1.2kb EcoR1(probe)

7) 400-----------------------1800 1.4 kb EcoR1 and BamH1(probe)

8)1000--------------1800 0.8kb BamH1 (probe)

9) 1000-----------1600 0.6kb BamH1 and EcoR1(probe)

10) 1600---1800 0.2kb EcoR1 and BamH1