in a TLC experiment, What is a retention factor? How do the polar properties of the separated molecules affect the retention factor?
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in a TLC experiment, What is a retention factor? How do the polar properties of the...
1. What is the retention factor for the following TLC plate if the baseline is 1 cm, elute solvent traveled 5cm, and the spot of interest traveled 2.5cm? a) 0.50 b) 1.6 c) 0.63 d) 1.4 10. Column chromatography employs both a stationary and a mobile phase. Which of the compounds listed below is commonly used as the stationary phase in column chromatography? a) decolonizing charcoal b) sodium bicarbonate c) benzoic acid d) silica gel
1. How does the sample separate on a TLC plate? 2.What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to visualize a TLC plate? 3.Describe how to obtain a Retention Factor. What does the retention factor tell you?
Calculate retardation factor (Rf) for two compounds separated by TLC and state which of the two are likely to be polar. (Explain your answer). Solvent front = 6.5 cm, Distance of A = 5.0 cm, Distance of B = 3.5 cm
In thin-layer chromatography, dhy do polar compounds require polar eluents to move them on the TLC plate?
1. A TLC experiment is performed to separate a mixture of the compounds shown below. 5.2 cm 4.8 cm • C 0-H O-H 2.3 cm • B OCH3 Phenol Cyclohexadiene m-anisic acid 1.1 cm • A a) Draw dipole arrows / dipole moments present for each molecule (if they have any) (2 points) b) The experiment yielded the TLC plate given on the left. Match each spot to one of the three molecules. For each compound, explain what makes it...
Table 2: Alcohol and Ketone Standards GC Retention Times GC#: Retention time Alcohol Standard Mixture Phent ion= 2.05Smin low Peak 1 Compound Name: athanol Rapesnal utanal aclapeatanal 4.755min oilng 6.895min .230 min Peak 2 Compound Name: Peak 3 Compound Name: Peak 4 Compound Name: GC#:1 Ketone Standard Mixture Retention time 2.575un Peak 1 Compound Name: Acclono Butanon a Pentanone 2tHex anon Peak 2 Compound Name: min Peak 3 Compound Name: 615 min 1D. 307 min Peak 4 Compound Name: Part...
what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer column is used.
Ok, so what would happen if you are working with TLC and have a polar stationary phase (silica) and a mobile phase ( polar eluting solution) and you spot a nonpolar compound? Would the Rf values be greater than in a nonpolar mobile phase? I know in general if you are using TLC with polar silica and spot a nonpolar compound the nonpolar compound travels further (higher Rf values) because its chemical structure is different than the stationary phase compared...
What would happen to the retention time of a compound if the following changes were made? Decrease the flow rate of the carrier gas Increase the temperature of the column Increase the length of the column How do the temperature and flow rate of the carrier gas affect the separation of two compounds that have been shown to separate by GC? 5. Rank the following compounds in terms of expected retention times on a non-polar GC Column TOH OCH.CH MW-114...
These questions are from an experiment where we had a mixture of Ferrocene, acetylferrocene and diacetyl ferrocene and we separated each by using column chromatography. Before doing the column chromatography we did TLC analysis to ensure that we did, in fact, have a mixture of these 3 compounds. During the column chromatography we first eluted the ferrocene using pure hexane. Then we eluted the acetylferrocene using a 1:1 mixture of hexane and methylene chloride. Then we eluted the diacetylferrocene using...