Question

1. more than 99% of the time, an intron ends with

a. GT B. AG. C. AT D. CG

2. The tagging enzyme used to produce 26bp of tags in SAGE analysis is :

a. BsmFI. b. Mmel. c. EcoP15I. d. none of the above

3. what is the metabolome of a cell ?

a. all of the proteins and nucleic acids of the cell

b. all of the mtabolities of the cell under a specific set of conditions

c. all of the potential metabolities that can e produced by a cell

d. all of the macromolecules of a cell

4. What is ORF?

a. all of the nucleotides of a gene that are transcribed into mRNA

b. the nucleotides of a gene that make up the codons specifying amino acids

c. the nucleotides of an mMRNA molecule before the introns have been removed

d. the amino acid sequence of a polypeptide

5. the enzyme combination used in SAGE analysis is:

a. AluI-BsmFI. b. EcoRI-TaqI. c EcoRI-MseI. d. None of the above

Answer 1

1. More than 99% of the time, an intron ends with AG, according to the GT-AG rule. Most splice sites of introns have similar sequences, starting with GT nucleotide and ending with AG nucleotide.

2. The tagging enzyme used to produce 26bp of tags in SAGE analysis is EcoP15I. SuperSAGE generates tags of 26 bp which have the tagging enzyme EcoP15I.

3. The metabolome of a cell is all of the potential metabolites that can be produced by a cell. The products of all the processes occuring in a cell gives a unique chemical fingerprint of the cell, which can be an important source of studying and analyzing the cell and its processes.

4. ORF refers to Open Reading Frame. These are the nucleotides of a gene that make up the codons specifying amino acids. It is a portion of a DNA molecule that, when translated into amino acids, contains no stop codons.

5. The enzyme combination used in SAGE analysis is EcoRI-TaqI. These are used for SuperSAGE analysis, where EcoRI is the tagging enzyme(TE) and TaqI is the anchoring enzyme(AE).