A mixture of arginine, glutamic acid, and leucine is applied to the center of an electrophoresis...
A mixture of arginine, glutamic acid, and leucine is applied to the center of an electrophoresis plate. After the pH is adjusted to 6.0 and an electric current is applied, the components of the mixture separate. Which of the following describes the plate after the experiment has been completed?
A. Leucine migrates towards the negative electrode, arginine migrates towards the positive electrode, and glutamic acid stays near the center.
B. Glutamic acid migrates towards the negative electrode, arginine migrates towards the positive electrode, and leucine stays near the center.
C. Arginine migrates towards the negative electrode, glutamic acid migrates towards the positive electrode, and leucine stays near the center.
D. Leucine migrates towards the negative electrode, glutamic acid migrates towards the positive electrode, and arginine stays near the center.
Homework Answers
Ans is C
Arginine has Isoelectric point value of 10.5 at pH 6 and the carboxyle group forms carboxilic acid -COOH and _NH2+,retain 1+ electrone So migrates towards the negative electrode,
glutamic acid has Isoelectric point value 3.2 at 6 pH -COOH renmains with 1 negative electon So migrates towards the positive electrode,
and leucine stays near the center.
Add Answer to:
A mixture of arginine, glutamic acid, and leucine is applied to
the center of an electrophoresis...
#1) For the following pentapeptide (Where pH =1): a) What would the net charge be for...
#1) For the following pentapeptide (Where pH =1):
a) What would the net charge be for the following pH?
pH 0 =
pH 3 =
pH 5 =
pH 7 =
pH 10 =
pH 13 =
b) Calculate the pl of the pentapeptide. (Cysteine: pK1 = 1.96,
pK2 = 10.128) ( Glutamine: pK1 = 2.17 , pK2 = 9.13) (Arginine: pK1
= 2.17. pK2 = 9.04) (Leucine: pK1 = 2.36 , pK2 = 9.60)
c) If you were to...
S Summeldild Du pole so that the DNA migrates to the center of the gel The...
S Summeldild Du pole so that the DNA migrates to the center of the gel The size standard should be near the negative pole and the DNA wells near the positive pole so that the DNA migrates to the center of the gel None of the above are correct Question 4 1 pts You set up a gel with the correct DNA and size standards in the correct well. The wells are placed near the positive pole and you turn...
c) You place your pentapeptide into a buffer with a pH=4. What direction will your pentapeptide...
c) You place your pentapeptide into a buffer with a pH=4. What direction will your pentapeptide move: Circle the correct answer. (2 marks) It will not move. Towards the positive electrode Towards the negative electrode Briefly Explain why: (2 marks) d) You are trying to separate 2 other peptides from your pentapeptide. Peptide 1 is made up of the amino acids: ARKS Peptide 2 is made up of the amino acids: ADES At what pH would you be able to...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively charged (with a charge of two electrons per base pair). When you “run the gel” you are generating an electric field by connecting anodes and cathodes at the ends of the gel. This causes the negatively charged DNA segments to move towards the positive electrode. After running the gel, smaller DNA segments have moved farther from the...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
Construct a flow chart describing the seperation of the mixture and the isolation of each compound...
Construct a flow chart describing the seperation of the
mixture and the isolation of each compound in this experiment. (Lab
steps/procedures includes for reference)
4. Construct a flow chart describing the separation of the mixture and the isolation of each compound in this experiment. A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. Most reactions of organic compounds require extraction at some stage of product purification. In this experiment you will use extraction...
d. Suppose you mistakenly acidify the filtrate with HCl instead of with HNO3. What would you...
d. Suppose you mistakenly acidify the filtrate with HCl instead of with HNO3. What would you obser after completing the halide test, and what would that observation lead you to conclude? Organic Laboratory Technique Primer Sodium Fusions and lon Testing Background: The elements that commonly occur in organic compounds along with carbon, hydrogen, and oxygen, are: nitrogen sulfur, and the halogens. The detection of these latter three elements depends on converting them into water soluble lons (CNS , and X...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has its own characteristic atom in which a. the atomic mass is constant. b. the atomic number is constant. c. the mass number is constant. d. Two of the above are correct. e. All of the above are correct. 2. Which of the following is not a trace element in the human body? a. iodine b. zinc c. iron d. calcium e. fluorine 3. A...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
#1) For the following pentapeptide (Where pH =1):
a) What would the net charge be for the following pH?
pH 0 =
pH 3 =
pH 5 =
pH 7 =
pH 10 =
pH 13 =
b) Calculate the pl of the pentapeptide. (Cysteine: pK1 = 1.96,
pK2 = 10.128) ( Glutamine: pK1 = 2.17 , pK2 = 9.13) (Arginine: pK1
= 2.17. pK2 = 9.04) (Leucine: pK1 = 2.36 , pK2 = 9.60)
c) If you were to...
S Summeldild Du pole so that the DNA migrates to the center of the gel The size standard should be near the negative pole and the DNA wells near the positive pole so that the DNA migrates to the center of the gel None of the above are correct Question 4 1 pts You set up a gel with the correct DNA and size standards in the correct well. The wells are placed near the positive pole and you turn...
c) You place your pentapeptide into a buffer with a pH=4. What direction will your pentapeptide move: Circle the correct answer. (2 marks) It will not move. Towards the positive electrode Towards the negative electrode Briefly Explain why: (2 marks) d) You are trying to separate 2 other peptides from your pentapeptide. Peptide 1 is made up of the amino acids: ARKS Peptide 2 is made up of the amino acids: ADES At what pH would you be able to...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
Construct a flow chart describing the seperation of the
mixture and the isolation of each compound in this experiment. (Lab
steps/procedures includes for reference)
4. Construct a flow chart describing the separation of the mixture and the isolation of each compound in this experiment. A commonly used method of separating a mixture of organic compounds is known as liquid-liquid extraction. Most reactions of organic compounds require extraction at some stage of product purification. In this experiment you will use extraction...
d. Suppose you mistakenly acidify the filtrate with HCl instead of with HNO3. What would you obser after completing the halide test, and what would that observation lead you to conclude? Organic Laboratory Technique Primer Sodium Fusions and lon Testing Background: The elements that commonly occur in organic compounds along with carbon, hydrogen, and oxygen, are: nitrogen sulfur, and the halogens. The detection of these latter three elements depends on converting them into water soluble lons (CNS , and X...
e. 18 Test Your Knowledge MULTIPLE CHOICE: Choose the one best answer. 1. Each element has its own characteristic atom in which a. the atomic mass is constant. b. the atomic number is constant. c. the mass number is constant. d. Two of the above are correct. e. All of the above are correct. 2. Which of the following is not a trace element in the human body? a. iodine b. zinc c. iron d. calcium e. fluorine 3. A...
Most questions answered within 3 hours.
-
A juice bottling company is carrying out a test at 10%
significance that the mean population...
asked 10 minutes ago -
What is the reflectivity (in percent) needed to keep water at a
temperature near its freezing...
asked 12 minutes ago -
Suppose that the average surface temperature of Earth is 300K.
Assume this is uniform over its...
asked 13 minutes ago -
Draw the condensed structural formula for alkenes and alkynes or
the line-angle structural formula for cycloalkenes...
asked 13 minutes ago -
M. Laing has suggested a new arrangement of the periodic table
(J.Chem.Ed.1989, 66,
746). Answer the following...
asked 34 minutes ago -
Café Michigan's manager, Gary Stark, suspects that demand for
mocha latte coffees depends on the price...
asked 33 minutes ago -
A US merchant purchases wine from a vineyard outside of Paris.
The wine is transported to...
asked 40 minutes ago -
we have (TTN)*
We have to use just one 2-bit counter with 1-bit global
history?
and...
asked 40 minutes ago -
Firms that fail to compete with lower prices and/or better
products will go bankrupt. Does this...
asked 41 minutes ago -
Question 1 Current and Resistance
Part A: A current of -14.5 nanoamperes is how many electrons...
asked 42 minutes ago -
The population standard deviation for the height of college
basketball players is 3.4 inches. If we...
asked 54 minutes ago -
6.) Use valence bond theory to describe the number and types of
hybrid bonding orbitals on...
asked 56 minutes ago