Homework Answers
Q4.a. DNA molecules is negatively charged because of the phosphate groups in their sugar-phosphate backbone. Thus they start moving towards the positive pole, run off the top of the gel. The gel will be blank.
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S Summeldild Du pole so that the DNA migrates to the center of the gel The...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
And.. Exercise1: Give the basic steps involved in extracting genomic DNA from animal cells and tossues?...
And..
Exercise1:
Give the basic steps involved in extracting genomic
DNA from animal cells and tossues?
23- Which of the following statements is correct? a. Longer DNA fragments migrate farther than shorter fragments. b. Migration distance is inversely proportional to the fragment size. c. Positively charged DNA migrates more rapidly than negatively charged DNA. d. Uncut DNA migrates farther than DNA cut with restriction enzymes. 24- Why do scientists load DNA of known sizes (also called "marker" or "ladder") into...
One strand of a DNA sequences is given below. Find the EcoRI sites and indicate the...
One strand of a DNA sequences is given below. Find the
EcoRI sites and indicate the cutting site with an arrow. Count the
number of bases in each fragment.
CP22: vne strand of a DNA sequence is given below. Find the EcoRI sites and indicate the cutting site with an arrow. Count the number of bases in each fragment. Restriction digest A: ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC Number of bases in each fragment: Now compare the same region of DNA from another individual. Where...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively charged (with a charge of two electrons per base pair). When you “run the gel” you are generating an electric field by connecting anodes and cathodes at the ends of the gel. This causes the negatively charged DNA segments to move towards the positive electrode. After running the gel, smaller DNA segments have moved farther from the...
A 1.2% Agarose Gel will separated DNA based on . O charge only both charge and size O size only n...
Please give clear answers along
with detailed explanations. Thank you so much!
A 1.2% Agarose Gel will separated DNA based on . O charge only both charge and size O size only neither charge nor size Question 2 A 1.2% Agarose Gel will separated dyes, proteins and amino acids based on . charge only both charge and size Osize only neither charge nor size Methylene Blue is a positively-charged dye. Which of the following components of DNA will it bind?...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
After PCR is performed the products are run out on an agarose gel. In the figure...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein...
Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein gel electrophoresis. Protein gel electrophoresis different composition. ) is carried out in the same manner as DNA gel electrophoresis (see Fig. 10.8) except the gel hasa 1. Sickle-cell hemoglobin (Hb) migrates more Figure 10.10 Gel slowly toward the positive pole than normal hemoglobin (Hb) because the amino acid valine has no polar R groups, whereas the amino acid +Pole glutamic acid does have a...
I am confused on this question, the correct answer is A and I chose B. I...
I am confused on this question, the correct answer is A and I
chose B. I thought the cathode was positively charged, and the
anode was negateively charged; and a high PI would incicate a more
positive charge, and a low PI would incidate a more negative chare.
Are these correct or am I backwards in some these? Also, are any of
these different for a galvanic or electrolytic cell?
Please explain how you would answer this question, thank
you!...
can you please answer all three questions. Thank you so much!! QUESTION 3: What is the...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
And..
Exercise1:
Give the basic steps involved in extracting genomic
DNA from animal cells and tossues?
23- Which of the following statements is correct? a. Longer DNA fragments migrate farther than shorter fragments. b. Migration distance is inversely proportional to the fragment size. c. Positively charged DNA migrates more rapidly than negatively charged DNA. d. Uncut DNA migrates farther than DNA cut with restriction enzymes. 24- Why do scientists load DNA of known sizes (also called "marker" or "ladder") into...
One strand of a DNA sequences is given below. Find the
EcoRI sites and indicate the cutting site with an arrow. Count the
number of bases in each fragment.
CP22: vne strand of a DNA sequence is given below. Find the EcoRI sites and indicate the cutting site with an arrow. Count the number of bases in each fragment. Restriction digest A: ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC Number of bases in each fragment: Now compare the same region of DNA from another individual. Where...
Please give clear answers along
with detailed explanations. Thank you so much!
A 1.2% Agarose Gel will separated DNA based on . O charge only both charge and size O size only neither charge nor size Question 2 A 1.2% Agarose Gel will separated dyes, proteins and amino acids based on . charge only both charge and size Osize only neither charge nor size Methylene Blue is a positively-charged dye. Which of the following components of DNA will it bind?...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein gel electrophoresis. Protein gel electrophoresis different composition. ) is carried out in the same manner as DNA gel electrophoresis (see Fig. 10.8) except the gel hasa 1. Sickle-cell hemoglobin (Hb) migrates more Figure 10.10 Gel slowly toward the positive pole than normal hemoglobin (Hb) because the amino acid valine has no polar R groups, whereas the amino acid +Pole glutamic acid does have a...
I am confused on this question, the correct answer is A and I
chose B. I thought the cathode was positively charged, and the
anode was negateively charged; and a high PI would incicate a more
positive charge, and a low PI would incidate a more negative chare.
Are these correct or am I backwards in some these? Also, are any of
these different for a galvanic or electrolytic cell?
Please explain how you would answer this question, thank
you!...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
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