Question

23- Which of the following statements is correct? a. Longer DNA fragments migrate farther than shorter fragments. b. Migration distance is inversely proportional to the fragment size. c. Positively charged DNA migrates more rapidly than negatively charged DNA. d. Uncut DNA migrates farther than DNA cut with restriction enzymes. 24- Why do scientists load DNA of known sizes (also called "marker" or "ladder") into the agarose gel? a. It makes it easier to determine sizes of unknowns using comparison techniques b. To fill in all the slots on the gel so you can run it c. To practice loading the DNA before you get to the important DNA b. So you know how long the gel needs to run 25 - Based on the following gel, what is the size of the band in lane 2?! a. 0.5:1.5 and 4 Kb b. 0.8; 1.9 and 4 Kb c.0.6; 1.6 and 4.2 Kb d. 0,6; 1 and 3.8 Kb IIIIIID- 26-Which of the following A260/280 ratios would be a reasonable cut off for RNA that you intend to use for quantitative RT-PCR? a. >1.8 b.31 c. 0.5 d. >3

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Exercise1:

Give the basic steps involved in extracting genomic DNA from animal cells and tossues?

Answer 1

Anstlers : is - B 23 Correct option Explanation: 1. Migration distance is inversly propostional to the Tragment Size. That is because larger foagments mare me to improve move through from the pores and Smalles fragments Can Easily pass the o and they can travel farther. pores hoc gh 24) Correct option is - A the detesmine size of technique. - It makes it easier to Knoons using Comparison Explanation:- 1. The bands in laddes show the size of DNA and when the band of unknown auns parallely to the laddess we can estimate the size of unknown Sample of DNA.

26 option - A is correct * 1 >1,8 Explanation": for pure RNA is generally accepted as cohich having A 260/280 ratio of 2.0.1.8 is quite commonly used..
for these {23,24,26} questions i gave correct answers and explanation too. i was tried to answer 25th question also but i didn't get exact answer for it. i hope so these three answers will helpful to you.

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