You are given a circular DNA molecule to analyze that is 3,000bp (3 Kb) long. You proceed to treat the DNA molecule with different cutting enzymes and then you run the individual reactions on a gel. The following gel is produced with each column representing a different reaction (lane). In lane 1 a molecule weight ladder is run. In lane 2, the circular DNA is NOT treated with any enzyme. In lane 3 the DNA is treated with an enzyme that cuts the circular molecule at 1 site only. Lanes 4 and 5 have reactions with enzymes that cut the circular DNA at 2 sites only.
Answer the following questions regarding the results of this
gel: a. The uncut DNA in lane 2 travels less than the expected 3Kb.
How could the difference between an uncut (circular) from the cut
(linear) DNA result in this effect? b. Considering that one cut
would result in the single band in lane 3, how would you justify
that the enzyme used for lane 4 ONLY cuts the circular molecule at
two sites? (Hint- add the number of base pairs for each band in
lane 4) c. You already know that the enzyme used in lane 5 will cut
the circular DNA in two places. However, unlike lane 4 you only
have one fragment. What does this tell about where the DNA is being
cut by the enzyme?
Homework Answers
Add Answer to:
You are given a circular DNA molecule to analyze that is 3,000bp
(3 Kb) long. You...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
Actual gels don't have labels. Here, the labels have been removed, but the ladder remains the...
Actual gels don't have labels. Here, the labels have been removed, but the ladder remains the same as in the previous example. 6. On the gel to the right, write the approximate size of each DNA fragment. Write the sizes next to each appropriate band. 7. Imagine that you have a sample of DNA that contains a single, specific DNA sequence. Before you run your gel, you split your sample into two tubes. You run the DNA from the first...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb in length. Lane "T" is a standard DNA ladder, not a digestion. (2 points total) I Gel lanes II III IV V Which of the following is TRUE? 20 K a) The enzyme used in lane III cuts 4 times b) The enzyme used in lane II cuts 1 time c) The enzyme used in lane IV cuts 3 times d) It is likely...
You are performing an analysis of squirrel mitochondrial DNA, which is a circular double-stranded DNA molecule...
You are performing an analysis of squirrel mitochondrial DNA, which is a circular double-stranded DNA molecule that is 23,000 bp in length. You are using restriction enzymes and agarose gel electrophoresis in your experiments. You have decided to use two restriction endonucleases: Pstl and Tagl. The picture below shows their recognition sites, and the red arrows indicate their strand- Pstl (Providencia stuartii) CTGCAG GACGTC specific cleavage sites. Taqi (Thermus aquaticus) TCGA AGCT Part A: The Pstl and Taql enzymes both...
Table 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA ...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
How did you EXPECT your uncut lane to look in the gel image? What ACTUALLY happened?...
How did you EXPECT your uncut lane to look in the gel image?
What ACTUALLY happened? What is a plausible explanation if there
was any discrepancy?
How did the observed BclI and EcoRI digest results compare to
the expected results? If they differed, list a potential reason
why?
BclI was incubated at 50°C, while EcoRI was incubated at 37°C.
Why was this necessary? What might you predict to occur if you
accidentally switched the temperatures?
3) Using the DNA ladder,...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments were produced of sizes 6 kb and 4 kb. The restriction enzyme HaeIII (H) also produced two fragments of sizes 9 kb and 1 kb. When the two enzymes HaeIII and EcoRI were used together, three fragments were produced of sizes 1 kb, 3 kb, and 6 kb. A 4 kb long cloned genomic probe from this region hybridized to the 3 kb and...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
Actual gels don't have labels. Here, the labels have been removed, but the ladder remains the same as in the previous example. 6. On the gel to the right, write the approximate size of each DNA fragment. Write the sizes next to each appropriate band. 7. Imagine that you have a sample of DNA that contains a single, specific DNA sequence. Before you run your gel, you split your sample into two tubes. You run the DNA from the first...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb in length. Lane "T" is a standard DNA ladder, not a digestion. (2 points total) I Gel lanes II III IV V Which of the following is TRUE? 20 K a) The enzyme used in lane III cuts 4 times b) The enzyme used in lane II cuts 1 time c) The enzyme used in lane IV cuts 3 times d) It is likely...
You are performing an analysis of squirrel mitochondrial DNA, which is a circular double-stranded DNA molecule that is 23,000 bp in length. You are using restriction enzymes and agarose gel electrophoresis in your experiments. You have decided to use two restriction endonucleases: Pstl and Tagl. The picture below shows their recognition sites, and the red arrows indicate their strand- Pstl (Providencia stuartii) CTGCAG GACGTC specific cleavage sites. Taqi (Thermus aquaticus) TCGA AGCT Part A: The Pstl and Taql enzymes both...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
How did you EXPECT your uncut lane to look in the gel image?
What ACTUALLY happened? What is a plausible explanation if there
was any discrepancy?
How did the observed BclI and EcoRI digest results compare to
the expected results? If they differed, list a potential reason
why?
BclI was incubated at 50°C, while EcoRI was incubated at 37°C.
Why was this necessary? What might you predict to occur if you
accidentally switched the temperatures?
3) Using the DNA ladder,...
Most questions answered within 3 hours.
-
In a performance test, each of two cars takes 8.9 s to
accelerate from rest to...
asked 57 seconds from now -
Sapphire Aerospace operates 52 weeks per year, and its cost of
goods sold last year was...
asked 2 minutes ago -
A. Suppose your manager indicates that for a normally
distributed data set you are analyzing, your...
asked 2 minutes ago -
For each the following molecules draw the best possible
structure based on formal charges for each...
asked 3 minutes ago -
A proton moves in the plane of this paper toward the top of the
page. A...
asked 12 minutes ago -
different divisions differing lines of business use
different costs of capital because their cost of equity...
asked 8 minutes ago -
Give an algorithm to determine whether or not the elements
of
an array of integers are...
asked 16 minutes ago -
. Gravimetric analysis. The gravimetric analysis that we will
perform in lab occurs according to the...
asked 34 minutes ago -
Learning curve problem:
A company was ordered to make 6 custom made parts. the labor
costs...
asked 32 minutes ago -
When you don’t recall operator precedences you can Guess
Experiment with the compiler Look in a...
asked 35 minutes ago -
GST – Complex issues:
Instructions: The following case study is related to complex tax
issues. You...
asked 38 minutes ago -
We are evaluating a project that costs $836,117, has an
eight-year life, and has no salvage...
asked 38 minutes ago