Question

HELP PLEASE! at least with some examples.

size of dna ladder- 1.8cm

Table 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF EACH DENTITY OF THE BAND SHAPE ToPOLOGY RNA LANE BAND IN THE LANE (Recombinant Plasmid, Plasmid Vector, (Circular (Relaxed oSIBLE? (Interpolated Values) Chromosomal DNA) Tobacco, or Bacterial r Linear) Supercoiled) (Y or N) (cm) Lane Lane Lane DNA Ladder Lane #4 Lane #5 Lane #6 Lane #7 Lane #8 Graph the known size (in bp) versus the distance migrated for each of the bands of the DNA ladder. Graph using semilog format as described in Procedure 6 Interpolate the size of molecules in each of the bands visible in lanes containing your DNA samples from your graph. Complete Table 8-3 indicating the size, shape, topology, and the identity of molecules in each band in the sample lanes of your gel.

Answer 1

For this question, measure the distance travelled by dna in each row with a scale that would give you distance in cm. For size of dna in each row, you need to know the size of dna ladder. The size of ladder is always in base pairs. Also smaller dna molecules will travel more distance than latger dna molecules. Wheather the dna is recombinant or plasmid can also be concluded from the size. Also the circular dna will run slowly as compared to linear dna. And also relaxed dna will run slowly as compared to supercoiled dna. Supercoiled dna will run faster than circular dna.