Question

TTC ach of the DNA fragments on your gel can be determined using another method th The number of base pairs in each of the DNA fragments the size of the known framments from the DNA marker against her method that can be more accurate. This involves graphing ents from the DNA marker against the distance each fough the gel to generate a standard curve. This is most DNA band moved through the gel, to generate a conveniently done on semilog graph paper. Look at the data from the practice gel on page 37. The frage size were plotted on semilog graph paper, producing the standar wactice gel on page 37. The fragments of known graph paper, producing the standard curve below. x-known fragment size o Unknown fragment size 3,000 Size, base pairs 7,000 6,000 5,000 4,000 Unknown fragment 1 Unknown fragment 2 3,000 2,000 1,000 Distance traveled, mm The distances migrated by two fragments of unknown length were also marked on the standard curve. 1. For each fragment, line up a ruler vertically from the distance traveled position on the horizontal X axis to the line that you constructed. 2. From the point where your ruler intersected your line, place the ruler horizontally and note where it intersects with the vertical Y axis for fragment size. This will be your determination of the size for that fragment. • How many base pairs is fragment 2? • How accurate is this estimation of size?

Answer 1

The size of the unknown fragment 2 according to the above graph is 4000 base pairs.

The estimation of size is 90% accurate.