Question

Clearly explain the role of BTB in the experiment of carbon fixation.

Answer 1

BTB: Bromothymolblue is an indicator used in experiment of carbon fixation. The indicator added to medium turns yellow in color when there is no carbon fixation and turns blue when there is carbon fixation.

An experiment is designed: 2 jars are taken with water. Air is blown through a straw. Prior air blowing BTB is added to one jar. The jar with BTB into which air is blown turns yellow when CO₂ enters into it.

Like wise 2 jars are taken with water and BTB. In one jar a plant is placed and kept in sunlight. When observed, the jar with palnt containing BTB turns back to blue because, the palnt absorbs CO₂ and fixes the carbon.

Place in light for one hour Elodea straw Bromothymol blue turns t0 yellow .U Water remains yellow Water turns blue