Q. A) An open tubular column is 30 m long and has an inner diameter of...
Q.
A) An open tubular column is 30 m long and has an inner diameter of 0.500 mm. It is coated on the inside wall with a layer of stationary phase which is 30 um thick. Unretained solute passed through in 2.00 min whereas a particular solute has a retention time of 15.20 min. What is the volume flow rate in mL/min.
B) Calculate the capacity factor for the solute.
C) What was the fraction of time spent in the stationary phase?
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A) An open tubular column is 30 m long and has an inner
diameter of...
An open tubular column with a diameter of 193 um and a stationary phase thickness on...
An open tubular column with a diameter of 193 um and a stationary phase thickness on the inner wall of 0.60 um passes unretained solute through in 57 s. A particular solute has a retention time of 485 s. What is the partition coefficient for this solute? K= What fraction of time does this solute spend in the stationary phase?
An open tubular column with a diameter of 187 um and a stationary phase thickness on...
An open tubular column with a diameter of 187 um and a stationary phase thickness on the inner wall of 0.88 um passes unretained solute through in 79 s. A particular solute has a retention time of 471 s (b) What fraction of time does this solute spend in the stationary phase? (a) What is the partition coefficient for this solute? Number Number 259.8 4.96 K= total
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm...
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
3. A capillary column for use in gas chromatography is 30.0 m long and has an...
3. A capillary column for use in gas chromatography is 30.0 m long and has an internal diameter of 0.320 mm. The inside wall of the capillary is coated with the stationary phase. a. The phase ratio (B) is the ratio of the mobile phase volume to the stationary phase volume: B = Vm/Vs. For a particular separation, the desired phase ratio is B = 200. What should the thickness of the stationary phase be to obtain this phase ratio?...
Need help with 5 Please The following questions 1-6 relate to the chromatogram shown below, obtained...
Need help with 5 Please
The following questions 1-6 relate to the chromatogram shown below, obtained in an experiment to separate 3 components in a mixture using a column of internal diameter 320.0 um (including the stationary phase thickness) and 30.0 m in length. The stationary phase thickness is 3.0 um on each side of the inside wall of the column, as shown in the figure. 320 um Detector Signal (UA) 0.6 1.2 1.8 Time (min) Unretained A 0.57 2.29...
An open tubular column with a diameter of 193 um and a stationary phase thickness on the inner wall of 0.60 um passes unretained solute through in 57 s. A particular solute has a retention time of 485 s. What is the partition coefficient for this solute? K= What fraction of time does this solute spend in the stationary phase?
An open tubular column with a diameter of 187 um and a stationary phase thickness on the inner wall of 0.88 um passes unretained solute through in 79 s. A particular solute has a retention time of 471 s (b) What fraction of time does this solute spend in the stationary phase? (a) What is the partition coefficient for this solute? Number Number 259.8 4.96 K= total
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
3. A capillary column for use in gas chromatography is 30.0 m long and has an internal diameter of 0.320 mm. The inside wall of the capillary is coated with the stationary phase. a. The phase ratio (B) is the ratio of the mobile phase volume to the stationary phase volume: B = Vm/Vs. For a particular separation, the desired phase ratio is B = 200. What should the thickness of the stationary phase be to obtain this phase ratio?...
Need help with 5 Please
The following questions 1-6 relate to the chromatogram shown below, obtained in an experiment to separate 3 components in a mixture using a column of internal diameter 320.0 um (including the stationary phase thickness) and 30.0 m in length. The stationary phase thickness is 3.0 um on each side of the inside wall of the column, as shown in the figure. 320 um Detector Signal (UA) 0.6 1.2 1.8 Time (min) Unretained A 0.57 2.29...
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