Homework Answers
Answer (a)
K = (471-79)/79 = 4.96
Answer (b)
The fraction of time = 471/(471+79) = 0.856
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An open tubular column with a diameter of 187 um and a stationary phase thickness on...
An open tubular column with a diameter of 193 um and a stationary phase thickness on...
An open tubular column with a diameter of 193 um and a stationary phase thickness on the inner wall of 0.60 um passes unretained solute through in 57 s. A particular solute has a retention time of 485 s. What is the partition coefficient for this solute? K= What fraction of time does this solute spend in the stationary phase?
Q. A) An open tubular column is 30 m long and has an inner diameter of...
Q. A) An open tubular column is 30 m long and has an inner diameter of 0.500 mm. It is coated on the inside wall with a layer of stationary phase which is 30 um thick. Unretained solute passed through in 2.00 min whereas a particular solute has a retention time of 15.20 min. What is the volume flow rate in mL/min. B) Calculate the capacity factor for the solute. C) What was the fraction of time spent in the...
A solvent passes through a chromatography column in 3.41 min, but the solute requires 9.13 min. What is the retenti...
A solvent passes through a chromatography column in 3.41 min, but the solute requires 9.13 min. What is the retention factor, k? k= What fraction of the time does the solute spend in the mobile phase in the column? fraction in mobile phase The volume of the stationary phase is 0.101 times the volume of the mobile phase in the column (V = 0.101V.). What is the partition coefficient, K, for this system?
A solvent passes through a chromatography column in 2.18 min, but the solute requires 8.45 min....
A solvent passes through a chromatography column in 2.18 min, but the solute requires 8.45 min. What is the retention factor, k? k= What fraction of the time does the solute spend in the mobile phase in the column? I fraction in mobile phase = The volume of the stationary phase is 0.102 times the volume of the mobile phase in the column (Vs = 0.102Vm). What is the partition coefficient, K, for this system? K=
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm...
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
A solvent passes through a chromatography column in 2.85 min, but the solute requires 9.43 min....
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An open tubular column with a diameter of 193 um and a stationary phase thickness on the inner wall of 0.60 um passes unretained solute through in 57 s. A particular solute has a retention time of 485 s. What is the partition coefficient for this solute? K= What fraction of time does this solute spend in the stationary phase?
A solvent passes through a chromatography column in 3.41 min, but the solute requires 9.13 min. What is the retention factor, k? k= What fraction of the time does the solute spend in the mobile phase in the column? fraction in mobile phase The volume of the stationary phase is 0.101 times the volume of the mobile phase in the column (V = 0.101V.). What is the partition coefficient, K, for this system?
A solvent passes through a chromatography column in 2.18 min, but the solute requires 8.45 min. What is the retention factor, k? k= What fraction of the time does the solute spend in the mobile phase in the column? I fraction in mobile phase = The volume of the stationary phase is 0.102 times the volume of the mobile phase in the column (Vs = 0.102Vm). What is the partition coefficient, K, for this system? K=
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
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