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Sodium chloride is a salt of strong acid (HCl) with strong base(NaOH). It does not hydrolyze in aqueous solution. Its aqueous solution is neutral with pH 7. pH of PBS is 7.4. It is different from pH 7. Hence, PBS will contain sodium chloride and a suitable buffer solution.
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3 Severely dehydrated patients may be given a "physiological buffered saline" (PBS) solution with a pH...
3. Severely dehydrated patients may be given a "physiological buffered saline" (PBS) solution with a pH of about 7.4 intravenously to restore their fluid balance. Do you think that PBS would contain only sodium chloride in the solution? Justify your answer. 4. Use the dilution equation to calculate the number of milliliters of 3.00 M NaCI needed to make 400.0 mL of a PBS solution with a final sodium chloride concentration of 0.154 M. Show your work. 5. Calculate the...
Severly dehydrated patients may be given a “physiological buffer saline” (PBS) solution with a pH of 7.4 intravenously to restore their fluid balance. Do you think that PBS would contain only sodium chloride in the solution? justify your answer.
Show calculations for the solutions. Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...