Solution :-
PBS does not contains only sodium chloride salt because sodium chloride is the neutral salt therefore the saline solution will have pH 7, but PBS saline is having pH 7.4 that means it is having basic pH therefore the PBS saline must contains some conjugate base salt of weak acid so that it can have basic pH.
So from the pH value we can confirm that along with sodium chloride some other salt would also present in the solution.
Severly dehydrated patients may be given a “physiological buffer saline” (PBS) solution with a pH of...
3 Severely dehydrated patients may be given a "physiological buffered saline" (PBS) solution with a pH of about 7.4 intravenously to restore thbeir fluid balance Do vou think that PBS would contain only sodium chloride in the solution? Justify your answer. www .4- Honda. Use the dilution equation to calculate the number of milliliters of 3.00 M NaCl needed to make 400.0 mL of a PBS solution with a final sodium chloride concentration of 0.154 M. Show your work. Calculate...
3. Severely dehydrated patients may be given a "physiological buffered saline" (PBS) solution with a pH of about 7.4 intravenously to restore their fluid balance. Do you think that PBS would contain only sodium chloride in the solution? Justify your answer. 4. Use the dilution equation to calculate the number of milliliters of 3.00 M NaCI needed to make 400.0 mL of a PBS solution with a final sodium chloride concentration of 0.154 M. Show your work. 5. Calculate the...
In lab, we have been using PBS. This is phosphate buffered saline. Phosphate Buffered Saline is usually 25mM to 100mM Phosphate Buffer with 137mM NaCl added and often at pH 7.4. You need to make 1L of 50mM PBS. The buffer is 50mM and the salt remains at 137mM. You have the following reagents on the shelf: - NaCl (MW = 58.44 g/mole); - Sodium phosphate dibasic heptahydrate (Na2HPO4 · 7H2O; MW = 268 g/mole) - Sodium phosphate monobasic...
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body. A) You need to prepare a stock solution at pH 7.00 with KH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2 ] + [H2PO4 − ] = 0.3...
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Pipene a sample into a well on a microplate and get an absorbance of 0.25, and your partner pipettes the same sample into the same plate and gets an absorbance of 0.32. Assume une microplate is clean and a blank well has been referenced properly. What could account for the difference in absorption readings? (Please reference Beer's Law in your answer.) 6. You are tasked with preparing 250 mL of a 100 mM sodium phosphate buffer (pKa - 7.21) containing...
Show calculations for the solutions. Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
Feedback Given that Kb 4.4x10-4 for methylamine exactly 650 mL of a buffer solution which has a pH of 10.84 and a final methylammonium chloride salt concentration [CHyNH,]-0.100 M, one would need to use (mass) of CH3NH3CI(s) Tries 0/S and (volume) of 3.00 M sodium hydroxide solution, plus enough water to make up the total volume. Answer Tries 0/5 Post Discussion Send Feed