Describe how you would make a solution of 50mM sodium chloride, 200 mM phosphate buffer, pH 6.5.
Describe how you would make a solution of 50mM sodium chloride, 200 mM phosphate buffer, pH...
3. How would you make 200 ml of a 250 mM phosphate buffer from a 1M stock solution of phosphate buffer? r. nus: how would you make 100 ul of 10 mM glucose solution from a 1M glucose stock solution? int: might need to do a serial dilution, as our pipettes cannot dispense less than 2ul)
1) How would you make 300 ml of a 0.1 M sodium phosphate buffer, pH 7.0 (pKa = 7.2) phosphate dibasic (the conjugate base). 2) What are the concentrations of acetate and acetic acid in a 0.2 M acetate buffer pH 5.3? The pKa for acetic acid is 4.76. 3)You have 100 ml of 0.1 M acetate buffer pH 5.2 (pKa 4.76). You add 10 ml of 0.1 M NaOH. Calculate the resulting change in pH and the buffering capacity...
Goal- to make a 1L solution of phosphate buffer at pH 7.8 If you make up a 1M aqueous solution each of: NaH2PO4 and Na2HPO4 How much of each solution would you need to add (mix) to make up a 1 liter solution of 0.4 M phosphate at pH 7.8 Hint: the pKa for H2PO4- <-> HPO4 2- + H+ is 6.8)
In a solution of 250 mM sodium phosphate buffer (so the concentration represents all forms of phosphate no matter the ionization state) at pH 5.2 (the pH of some synaptic vessicles), what is the ratio of H2PO4- to HPO4-2 ? The pKa's of phosporic acid are 2.15, 6.86 and 12.32. Set up the equation to solve for the answer (you aren't expected to give a numerical answer). Is this an appropriate buffer at this pH? Why or why not?
3. Describe, quantitatively, how you would make 250 mL of a 40 mM HEPES buffer at pH 7.60. You have access to HEPES sodium salt (molar mass = 260.29), unlimited 1 M NaOH and unlimited 1 M HCl.
How you would prepare a solution of 250.0 mL of a 0.150M phosphate buffer at ph=7. (phosphoric acid pka1=2.12, pka2=7.21, pka3=12.32). What would the pH of the buffer be if 5.00mL of 1.10M HCL was added to 100mL sample of the prepared buffer.
A student was told to make a pH = 7.5 buffer from sodium dihydrogen phosphate and sodium hydrogen phosphate with an ionic strength of 0.10 a) What concentration of Na2HPO4 and NaH2PO4 need to be used? b) If 10.0 mL of 1.00 M Hbr is added to 1.00 L of this buffer, what is the pH? Please show work.
You need to make 1 L of 100 mM acetate buffer at pH 5. But oh no! The pH meter is broken, and you won’t be able to measure the pH of your solution. You look around on the shelves in lab and find a 100 mM solution of acetic acid (pKa = 4.76) and a 100 mM solution of sodium acetate. How do you make your buffer? Show your work / reasoning.
How would you prepare 200 mL of 20 mM NaH2PO4 buffer, pH = 7.0?
James wants to make a 6% sodium chloride solution using water as a buffer. If the final volume of the solution is 2000 mL, how much sodium chloride does James need?