How would you prepare 200 mL of 20 mM NaH2PO4 buffer, pH = 7.0?
Molarity= Mass in grams/ molecular weight * 1000/vol req
20/1000 = x/119.98 * 1000/ 200
x= 20/1000 * 119.98 *200/1000
=0.47g of NaH2PO4 in 200mL of d.H2O
Check the pH and adjust it using either acid (HCl) or base (NaOH)
how would you prepare 300ml of 20mM NaPO4 buffer, pH=7.0
a) What are the concentrations of Na2HPO4 and NaH2PO4 in a 0.30 M phosphate buffer solution pH 7.0? Use pKa 6.82 b) Describe how you would prepare 250 mL of the buffer in part (a) given that you have available to you a 1.0 M stock solution of NaH2PO4 and solid Na2HPO4(FW 141.96 g/mol). Provide your answer in milliliters of NaH2PO4 and grams of Na2HPO4. c) Suppose you use 100 ml of this buffer in an experiment and 0.003 mol...
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1) Solution Components A 1 mL 100 mM NaH2PO4 + 9 mL 100 mM Na2HPO4 B 5 mL 100 mM NaH2PO4 + 5 mL 100 mM Na2HPO4 C 9 mL 100 mM NaH2PO4 + 1 mL 100 mM Na2HPO4 D 10 mL 100 mM NaH2PO4 stock solution E 10 mL 100 mM Na2HPO4 stock solution F 10 mL distilled water pKa of phosphate: 6.8 How would you calculate the pH of each equation using the Henderson Hasselbalch equations? 2) Tris...
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