3. Describe, quantitatively, how you would make 250 mL of a 40 mM HEPES buffer at...

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3. Describe, quantitatively, how you would make 250 mL of a 40 mM HEPES buffer at...

3. Describe, quantitatively, how you would make 250 mL of a 40 mM HEPES buffer at pH 7.60. You have access to HEPES sodium salt (molar mass = 260.29), unlimited 1 M NaOH and unlimited 1 M HCl.

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Answer 1

Answer #1

Molecular weight of HEPES-Na salt = 260.29gmol^-1

Concentration of the buffer to be prepared = 40mM or 40 * 10^-3M

Volume of the solution to be made = 250mL

Procedure:

Dissolve 2.6029g in 100mL distilled water.
Adjust the pH to 7.6 using HCl and NaOH.
Once after the pH is adjusted, add the distilled water to make the volume to 250mL.

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Answer 2

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