How would you prepare a 105 mL solution of 2.2% HEPES from a 250 mM stock...
3. Describe, quantitatively, how you would make 250 mL of a 40 mM HEPES buffer at pH 7.60. You have access to HEPES sodium salt (molar mass = 260.29), unlimited 1 M NaOH and unlimited 1 M HCl.
3. How would you make 200 ml of a 250 mM phosphate buffer from a 1M stock solution of phosphate buffer? r. nus: how would you make 100 ul of 10 mM glucose solution from a 1M glucose stock solution? int: might need to do a serial dilution, as our pipettes cannot dispense less than 2ul)
You are asked to prepare 500 ml of a 0 250 M acetate Puffer at pH 5.00 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76). 3 00 M NaOH, water. Answer the following questions regarding the preparation of the buffer. How many grams of acetic acid will you need to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. What volume of 3.00...
19. a. What mass of potassium bromide is needed to prepare a 250. mL of a 2.00 M KBr aqueous solution? Molar mass of KBr = 119.00 g/mol. Use dimensional analysis to calculate the mass. b. Describe the full process of how you would prepare this solution in a 250. mL volumetric flask. c. Calculate the concentration of the solution in question a. if more water was added to bring the solution to a final volume of 1000. mL. (Hint:...
10. From a 3.01 mg/mL stock solution of ONPG (MW 301.25), prepare 1000 ML of a 1.0 mM ONPG solution. How much stock and how much water are needed to make this solution? Show your work. Be sure to include proper units!
1) Figure below show a 36 uL of stock solution containing 800 ppm of CuSO4 was transferred into T1 tube contained with 324 uL of wash buffer. Next, serial dilution was performed from T1 to T5. (CuSO4 molar mass: 159.61 g/mol) 36 ul 120 ML 120 ML 120 L 120 L 800 ppm Cuso T1 Stock solution T2 T3 T4 TS B1 Dilution Factor Final Concentration NA 800 ppm Tube Volume Diluent Total Final Transferred Volume Volume Volume (PL) (wash...
You are asked to prepare 500 mL 0.300 M acetate buffer at pH 4.90 using only pure acetic acid (MW = 60.05 g/mol pk, = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: What volume of 3.00 M NaOH, must be added to the acetic acid to achieve a...
For Experiment 2 you need to prepare a 10X dilution of a 100 mM NaCl stock solution (Mr 58.44). What is the concentration of the dilution in mg/mL?
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.10 using only pure acetic acid (MW = 60.05 g/mol, pka = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: DD What volume of 3.00 M NaOH, must be added to the acetic acid to achieve...
You are asked to prepare 500. mL 0.300 M acetate buffer at pH 5.10 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: 60 What volume of 3.00 M NaOH, must be added to the acetic acid to achieve...