Question

5. How would you prepare a 105 mL solution of 2.2% HEPES from a 250 mM stock of the buffer? (MW of HEPES 238.30) (6 pts) a) First, convert 250 mM HEPES to a percent concentration b) Next, determine the dilution of your percent stock solution of HEPES (calculated in a above) to a final concentration of 2.2% with a final volume of 105 ml c) Mix components ml of H20 ml of 250 mM HEPES
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Answer #1

A) 1000mL 250mM in % means in 100mL 250mM * 100 / 1000 25mM or 25% V1M1 105 * 2.2% 105 * 2.2 /25 B) V2M2 V2 25 V2 9.24 mL enc

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