Question

mol gen

A. Clearly and simply draw recently synthesized DNA strands (including at least 1 Okazaki fragment per lication "bubble" on the left side in the figure below. Accurately and carefully mark fork) associated with the repl the 5' and 3' ends of each strand and state what the dotted lines shown below correspond to. [6 p oints) RE RE 5' 3' 2D-gel probe

Answer 1

DNA replication always takes place in 5' to 3' direction. So, on 3' to 5' parent strand , this replication is very easy as the synthesis can take place easily from 5' to 3'. But on the other parent strand which is from 5' to 3' in direction. For this many short fragments of around 1000 base pairs , called Okazaki fragments are formed in 5' to 3' direction(as labelled in the figure). These okazaki fragments are then joined together to form a complete strand by the enzyme DNA ligase.

3 5 LAGGING <-- ORIGIN OF REPLICATION STRAND OKAZAKI PRAG MENT 3 5 51 3 3 5 3 PRIMERS →LEADING STRAND EXPANDED FICURE)