In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands.
a. To which direction (left or right) is the replication fork is moving? (1 mark)
b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks)
c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments? (1 mark)
(ii) What is the difference of this enzyme from the enzyme synthesizing leading strand? (2 marks)
d. Which enzyme in E. coli removes the primers? (1 mark)
Homework Answers
A. The replication fork is moving towards the right . Because the new strand segments in towards the right of the boxes(primer ) .
B. As we know that the replication fork is moving towards the right, the A segment is made before the B segment.
C.i) the enzyme that forms the primer is called Primase . It is a type of RNA polymerase .
ii) The leading strand is formed in the 5' to 3' end continuously with the help of DNA polymerase .
On the other hand the lagging strand is formed in the 3'to 5' end . Enzyme is Primase .
D. Primers are removed by DNA polymerase I .
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In the following diagram, A and B are two Okazaki fragments
generated during DNA replication. Open...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
Draw a figure of one double stranded DNA molecule that has initiated replication and produced two...
Draw a figure of one double stranded DNA molecule that has initiated replication and produced two okazaki fragment in each lagging strand. The figure must include both template strands, all newly synthesized DNA molecules on both leading and lagging strands, all RNA primers, direction of chain growth for each fragment/strand indicated with arrowheads, direction of replication forks. Each molecule must be labeled with the origin of replication on both strands, leading and lagging strands labeled, template or newly synthesized, DNA...
Vocabulary: DNA Replication
Vocabulary: DNA Replication A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase .1. Site where the replication of a DNA molecule begins. 2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 4. Relaxes...
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the...
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the formation of Okazaki fragments in the leading strand. (C) is a consequence of replicating both strands of template DNA at a single replication fork. (D) requires its own replisome.
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase...
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase is required to add new nucleotides to the growing ends of the DNA strands. DNA ligase joins the small DNA fragments of the lagging strand. Only one strand of the parent DNA serves as a template for a newly synthesized complementary strand.
2) (2 pts) The diagram below depicts two Okazaki fragments synthesized during lagging strand DNA replication....
2) (2 pts) The diagram below depicts two Okazaki fragments synthesized during lagging strand DNA replication. Which arrow indicates the position at which the synthesis of the next RNA primer will be initiated? Circle the correct letter 3 5 Template strand 3) (2 pts) The E. coli genome encodes 3 different DNA polymerases, namely, L,II and II. a) Which one has 5' to 3' exonuclease activity? Why? b) Which one is most abundant?
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
11. Several enzymes and proteins participate in DNA replication. Answer the fill in the blan below...
11. Several enzymes and proteins participate in DNA replication. Answer the fill in the blan below bonds unwinds DNA by breaking the a. The enzyme between the nitrogenous bases bind to single-stranded DNA to stabilize it and to prevent it from reannealing to the other DNA strand. makes DNA This enzyme adds nuceotides to the end of a nucleic acid strands; therefore, it makes DNA in the to direction . DNA Polymerase cannot put two nucleotides together, instead, it adds...
larger photo is for reference. only need to answer part d ODOLOUUU POLO See above -...
larger photo is for reference. only need to answer part
d
ODOLOUUU POLO See above - In the synthesis of the DNA molecule in (a-c) how many primers are generated? Explain. 11. Below is a DNA replication fork. Leading Strand 32 بن 5'_ t rading HR3 3 a lagging strands U . 2GB 52) A 14 a. On the diagram label the 5' and 3' end of the parental DNA strands. (4 points) b. Which parental DNA strand (top or...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
2) (2 pts) The diagram below depicts two Okazaki fragments synthesized during lagging strand DNA replication. Which arrow indicates the position at which the synthesis of the next RNA primer will be initiated? Circle the correct letter 3 5 Template strand 3) (2 pts) The E. coli genome encodes 3 different DNA polymerases, namely, L,II and II. a) Which one has 5' to 3' exonuclease activity? Why? b) Which one is most abundant?
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
11. Several enzymes and proteins participate in DNA replication. Answer the fill in the blan below bonds unwinds DNA by breaking the a. The enzyme between the nitrogenous bases bind to single-stranded DNA to stabilize it and to prevent it from reannealing to the other DNA strand. makes DNA This enzyme adds nuceotides to the end of a nucleic acid strands; therefore, it makes DNA in the to direction . DNA Polymerase cannot put two nucleotides together, instead, it adds...
larger photo is for reference. only need to answer part
d
ODOLOUUU POLO See above - In the synthesis of the DNA molecule in (a-c) how many primers are generated? Explain. 11. Below is a DNA replication fork. Leading Strand 32 بن 5'_ t rading HR3 3 a lagging strands U . 2GB 52) A 14 a. On the diagram label the 5' and 3' end of the parental DNA strands. (4 points) b. Which parental DNA strand (top or...
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