To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse...
To prepare the protein sample, you need mix proteins and Laemmli buffer (5x) and then fuse water to make up to 15ml . You have 4ml protein sample, calculate how many water and Lammeli Buffer you need to add? Laemmli final concentration should be 1x.
Homework Answers
Final volume is 15 more and final concentration of buffer is 1x.
Initial concentration of buffer is 5x.
Using dilution law
MfVf = MiVi
Vi = 1x × 15/ 5x = 3 mL
Now you have to take 3 ml laemmli buffer, 4 ml protein sample and 15 - 3- 4 = 8 ml water for final concentration.
Add Answer to:
To prepare the protein sample, you need mix proteins and Laemmli
buffer (5x) and then fuse...
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below. Sample Protein Concentration mg/mL Protein Sample (microliter) Water (microliter) 4X Sample Buffer (microliter) Total Sample Volume (microliter) Total Lysate (-) 1 mg/mL 20 E1 0.07 mg/mL 20 E2 0.15 mg/mL 20 E3 0.2 mg/mL 20
Prepare the buffer. In this lab we store our buffer at a concentration 20 times the...
Prepare the buffer. In this lab we store our buffer at a concentration 20 times the concentration used in the experiment. Thus we call it 20xLB. Before we can use it, it needs to be diluted to a 1x concentration. If we needed 20 mL of the media, we would take 1 mL of the 20xLB buffer and diluted it with 19 mL of Dl water. To make enough buffer for this experiment, we need 50 mL for the gel...
You are asked to prepare 500. mL of a 0.100 M acetate buffer at pH 5.00 using only pure acetic acid (MW=60.05 g/mol,...
You are asked to prepare 500. mL of a 0.100 M acetate buffer at pH 5.00 using only pure acetic acid (MW=60.05 g/mol, pKa=4.76), 3.00 M NaOH, and water. Answer the following questions regarding the preparation of the buffer. 1. How many grams of acetic acid will you need to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. 2. What volume of 3.00 M NaOH must...
a) You want to load 15 ug of protein in 10 uL into one of the...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.10 using only...
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.10 using only pure acetic acid (MW = 60.05 g/mol, pka = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: DD What volume of 3.00 M NaOH, must be added to the acetic acid to achieve...
You are asked to prepare 500. mL 0.300 M acetate buffer at pH 5.10 using only...
You are asked to prepare 500. mL 0.300 M acetate buffer at pH 5.10 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: 60 What volume of 3.00 M NaOH, must be added to the acetic acid to achieve...
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.00 using only...
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.00 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetat refers to the concentration of all acetate species in solution. mass: What volume of 3.00 M NaOH, must be added to the acetic acid to achieve a...
You are asked to prepare 500 mL 0.300 M acetate buffer at pH 4.90 using only...
You are asked to prepare 500 mL 0.300 M acetate buffer at pH 4.90 using only pure acetic acid (MW = 60.05 g/mol pk, = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: What volume of 3.00 M NaOH, must be added to the acetic acid to achieve a...
1.- Mark has received a very expensive protein that may be critical in the discovery for...
1.- Mark has received a very expensive protein that may be critical in the discovery for cancer treatment. Before running his experiment, he remembers his protein’s concentration is labeled “50X”. Mark wishes to utilize a final volume of 200 µL. By what dilution factor must Mark dilute his expensive protein to be in working conditions (1X)? How many parts of protein and buffer make up this dilution? B. How much volume (in µL) of protein and buffer would Mark...
You are asked to prepare 500 ml of a 0 250 M acetate Puffer at pH...
You are asked to prepare 500 ml of a 0 250 M acetate Puffer at pH 5.00 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76). 3 00 M NaOH, water. Answer the following questions regarding the preparation of the buffer. How many grams of acetic acid will you need to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. What volume of 3.00...
Prepare the buffer. In this lab we store our buffer at a concentration 20 times the concentration used in the experiment. Thus we call it 20xLB. Before we can use it, it needs to be diluted to a 1x concentration. If we needed 20 mL of the media, we would take 1 mL of the 20xLB buffer and diluted it with 19 mL of Dl water. To make enough buffer for this experiment, we need 50 mL for the gel...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.10 using only pure acetic acid (MW = 60.05 g/mol, pka = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: DD What volume of 3.00 M NaOH, must be added to the acetic acid to achieve...
You are asked to prepare 500. mL 0.300 M acetate buffer at pH 5.10 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: 60 What volume of 3.00 M NaOH, must be added to the acetic acid to achieve...
You are asked to prepare 500. mL 0.150 M acetate buffer at pH 5.00 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500. mL buffer? Note that the given concentration of acetat refers to the concentration of all acetate species in solution. mass: What volume of 3.00 M NaOH, must be added to the acetic acid to achieve a...
You are asked to prepare 500 mL 0.300 M acetate buffer at pH 4.90 using only pure acetic acid (MW = 60.05 g/mol pk, = 4.76), 3.00 M NaOH, and water. How many grams of acetic acid will be needed to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. mass: What volume of 3.00 M NaOH, must be added to the acetic acid to achieve a...
You are asked to prepare 500 ml of a 0 250 M acetate Puffer at pH 5.00 using only pure acetic acid (MW = 60.05 g/mol, pKa = 4.76). 3 00 M NaOH, water. Answer the following questions regarding the preparation of the buffer. How many grams of acetic acid will you need to prepare the 500 mL buffer? Note that the given concentration of acetate refers to the concentration of all acetate species in solution. What volume of 3.00...
Most questions answered within 3 hours.
-
Starting with benzene, synthesize 1-phenyl-1-butyne.
Show intermediates and reagents.
asked 47 minutes ago -
Create a 32-run crossed array design with six control factors
and two noise factors such that...
asked 1 hour ago -
A 500g sample of sand from source A has the following amounts
retained on each sieve....
asked 1 hour ago -
In
your own words, please explain the essay by John Keynes wrote "The
End of Laissez...
asked 1 hour ago -
How are the matrix and pixels related? Why are smaller
pixels better for diagnostic quality?
asked 1 hour ago -
2. An AC generator has 80 rectangular loops on
its armature. Each loop is 11 cm...
asked 1 hour ago -
Please help me with this question. Consider Aldi’s current and
potential geographic markets (see Exhibit 4...
asked 1 hour ago -
What are the main components of the fermentation process and
give an explanation of each? Include...
asked 2 hours ago -
Explain which types of cells in the body (belonging to which
organs, etc.) are sensitive to...
asked 2 hours ago -
A single cable supports an 703-kg elevator car. What is the
tension in the cable when...
asked 2 hours ago -
among the three different ways to link CSS specifications to an
HTML document (inline CSS, document...
asked 2 hours ago -
(1) Write the net ionic equation for the reaction that occurs
when equal volumes of 0.191...
asked 2 hours ago