what type of column would NOT be suitable for the separation of o-chlorophenol and p-chlorophenol (...
what type of column would NOT be suitable for the separation of o-chlorophenol and p-chlorophenol ( reverse phase hplc, size exclusion, normal phase hplc) ?
Homework Answers
Size exclusion chromatography will not be used as SEC is used to separate big molecular weight molecules from the small molecular weight molecules I.e. SEC is used based on molecular weigh and not for separation of positional isomers as above. ,
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what type of column would NOT be suitable for the separation of
o-chlorophenol and p-chlorophenol (...
1. Select an appropriate type of column for separating these steroids and give the rationale for...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
Each of the following statements describes a type of column chromatography. Sort the statements to the...
Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.) Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All Statements: Separate molecules by size. Separate molecules by charge. The stationary phase has a covalently bound group to which a protein...
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00,...
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00, circle which of the following compounds would elute first and briefly describe why: Propanoic acid (CH3CH2COOH, PK. = 4.87) or Propylamine (CH2CH2CH2NH2, pks = 3.43). b. If one uses gradient elution with normal phase chromatography, would it be better to go from a less polar to a more polar solvent or a more polar to a less polar solvent? Why?
9. a) Draw a block diagram for a High Performance Liquid Chromatograph (HPLC), showing all the...
9. a) Draw a block diagram for a High Performance Liquid Chromatograph (HPLC), showing all the important components. Briefly describe the function of each part. b) List four types of stationary phase/separation methods that are available for HPLC and suggest suitable analytes or analyses for each type of stationary phase. c) List four detection options that exist for HPLC and discuss the good and bad points (if any) of each. d) Choose one aspects of chromatography (eg. resolution or sample...
Hello all, we did a lab about column chromatography, objective was Separation of a Fluorene and...
Hello all, we did a lab about column chromatography, objective
was Separation of a Fluorene and Fluorenol mixture.
Please answer the following (do not write in cursive):
Introduction and Background: Introduce technique of normal phase column chromatography and thin-layer chromatography. O Purpose o The principle of how they work – include the mobile and stationary phases o Rf value O Applications
(3) During the separation of two steroids by HPLC on a C18 column, a student reported the following elution times with...
(3) During the separation of two steroids by HPLC on a C18 column, a student reported the following elution times with a certain % acetonitrile/water mobile phase (uracil was added to the steroid mixture) tm (uracil) 1.2 min t, (steroid1) 4.55 min t, (steroid2) 6.55 min base width, w (steroid 1) 0.56 min base width, w (steroid 2) 0.79 min a. Sketch the chromatogram to illustrate the above separation. Label the peaks and the x- and y-axis. Calculate the resolution...
What effect will the following factors have on a column chromatographic separation with alumina as the...
What effect will the following factors have on a column chromatographic separation with alumina as the stationary phase? A) methanol is used as the initial eluting solvent B) The level of the eluting solvent falls below the top of the column? C) Heptane is used as the initial eluting solvent
Question 1: (column chromatography) a. What effect on the chromatographic separation is expected ...
Question 1: (column chromatography) a. What effect on the chromatographic separation is expected if solvent level is below the adsorbent? b. Once the chromatographic column was prepared, why was it important to allow the level of the liquid in the column to drop to the level of the stationary phase before applying the solution of the compounds to be separated? c. Name two methods of carrying out the column chromatography technique? d. What are the two methods of packing a...
4) What is the order of elution of the following compounds from a reverse-phase packed HPLC...
4) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C6H5CH2OH), benzaldehyde (C6H5CHO), benzoic acid (C6H5COOH), Acetophenone (C6H5C(O)CH3)
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
1. Select an appropriate type of column for separating these steroids and give the rationale for your selection. HO HO ОН OH Prednisolone Prednisone 2. You are using a C18 Reverse Phase column and the parameters shown below to separate Acetaminophen, Caffeine, and Benzoic Acid. You observe three peaks in your chromatogram (Huzzah!). Which corresponds to which compound? Explain the rationale behind your assignment. OH C18 REVERSED PHASE COLUMN Acetaminophen HC N H, O Caffeine Column: Legacy L1 Size: 4.6...
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00, circle which of the following compounds would elute first and briefly describe why: Propanoic acid (CH3CH2COOH, PK. = 4.87) or Propylamine (CH2CH2CH2NH2, pks = 3.43). b. If one uses gradient elution with normal phase chromatography, would it be better to go from a less polar to a more polar solvent or a more polar to a less polar solvent? Why?
Hello all, we did a lab about column chromatography, objective
was Separation of a Fluorene and Fluorenol mixture.
Please answer the following (do not write in cursive):
Introduction and Background: Introduce technique of normal phase column chromatography and thin-layer chromatography. O Purpose o The principle of how they work – include the mobile and stationary phases o Rf value O Applications
(3) During the separation of two steroids by HPLC on a C18 column, a student reported the following elution times with a certain % acetonitrile/water mobile phase (uracil was added to the steroid mixture) tm (uracil) 1.2 min t, (steroid1) 4.55 min t, (steroid2) 6.55 min base width, w (steroid 1) 0.56 min base width, w (steroid 2) 0.79 min a. Sketch the chromatogram to illustrate the above separation. Label the peaks and the x- and y-axis. Calculate the resolution...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
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