Question

Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.)

Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All

Statements:

Separate molecules by size.

Separate molecules by charge.

The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.

Uses a mobile phase and a stationary phase to separate proteins.

The stationary phase contains cross-linked polymers with different pore sizes.

Can separate molecules based on protein-lignand binding.

The stationary phase may contain negatively or positively charged groups.

Answer 1

Statements:

Separate molecules by size. - Size exclusion

Separate molecules by charge. - ion exchange

The stationary phase has a covalently bound group to which a protein in the mobile phase can bind. - affinity

Uses a mobile phase and a stationary phase to separate proteins. - All

The stationary phase contains cross-linked polymers with different pore sizes. - Size exclusion

Can separate molecules based on protein-lignand binding. - affinity

The stationary phase may contain negatively or positively charged groups. - ion exchange