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ANSWER ALL 1) Which of the following is the stationary phase in size exclusion chromatography? A....

ANSWER ALL

1) Which of the following is the stationary phase in size exclusion chromatography?

A. resin

B. mixture of proteins

C. solvent or buffer

D. none of these

2) Suppose you wish to purify a protein that has many positively charged residues from a mixture of proteins.

Which of the following columns would elute the protein the fastest?

A. column packed with neutral resin

B. column packed with positively charged resin

C. column packed with negatively charged resin

D. column packed with polar resin

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Answer #1

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Answer #2

1.The stationary phase in size exclusion chromatography is A. resin.

2.The column that would elute the protein the fastest is C. column packed with negatively charged resin. Proteins with many positively charged residues are attracted to negatively charged resins and will therefore bind strongly to the resin. To elute the protein, a higher concentration of salt or a more extreme pH can be used to disrupt the electrostatic interactions between the protein and the resin.


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