Question

Match the following terms with correct definitions An anion exchanger column Gel filtration column An affinity resin A exchanger column Packed or poured column An insoluble stationary phase to which molecule are attached (or immobilized) A resin that has negatively charged functional groups Column is filled with absorbing material An immobile matrix designed to separate molecules on the basis of molecular weight A resin that has positively charged functional groups Choose the appropriate resin to separate individual protein in the following mixture the size of the molecule (1)-MW: 3 kD, pi: 1; (2)MW: 20 KD, pl: 6.8; and (3) MW: 40 KD. pi: G-25, Fractionation range:1-5 KD G-75, Fractionation range:3-80 KD G-100, fractionation range:4-150 KD DEAE-spandex

Answer 1

Hi, below are the explained answers for the matching problem.

a) An anion exchanger column --> 26.Resin that has positively charged fuctional group. This is because Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE).

b) Gel Filtration column --> 25. An immobile matrix designed to separate molecules on the basis of molecular weight. This is because Gel filtration chromatography seprarates proteins, peptides, and oligonucleotides on the basis of size. Molecules move through a bed of porous beads, diffusing into the beads to greater or lesser degrees. Smaller molecules diffuse further into the pores of the beads and therefore move through the bed more slowly, while larger molecules enter less or not at all and thus move through the bed more quickly. Both molecular weight and three-dimensional shape contribute to the degree of retention.

c) An affinity Resin --> 22. An insoluble stationary phase to which a ligand molecule is attached. This is because Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand.

d) A cation exhanger column --> 23. A resin that has negatively charged functional group. Remember that the net charge of the molecule(s) of interest determines resin choice. If positively charged molecules are to be immobilized by the column resin, a cation exchange resin is chosen, whereas a positively charged anion exchange resin is chosen if negatively charged molecules are to be captured by the resin.

e) Packed or poured column --> 24.Column is filled with absorbing material. Packed columns contain a finely divided, inert, solid support material coated with liquid stationary phase. This liquid stationary phase material is the one which causes absorption of material.

I am sorry as I will not be able to do next questions as question seems to be incomplete due to the improper picture but I hope the explnation above should have helped.