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At ph -7, the amino acids D G and Rare seperated using anion exchange chromotography, which...
The five proteins listed below were run on a cation exchange column at pH = 7 Cytochrome C pl = 10.7 Ribonuclease A pl = 9.5 Myoglobin pl = 7.4 Carbonic anhydrase pl = 6.0 Glucose oxidase pl = 4.2 Which of the following statements is TRUE? A) Glucose oxidase and carbonic anhydrase will be separated in the low salt wash b) Cytochrome C binds and then elutes first when the salt concentration is increased c) decreasing the pH will...
The order of amino acids (Lys, Asp, ILE) eluted from an anion exchange column at PH 7 should be: KDI DIK DKI IKD no correct answer Please explain why the answer is no correct answer. Thank you.
15. Which is used to reduce the disulfide bonds in proteins? D) iodoacetate B) performic acid C) DTT A) PITC 16. What is the net charge on the tripeptide Gly-Asp-Lys at pH 1.5? The table below gives the pKa's of the ionizable groups on the free amino acids. Free amino acid pK, of a-Carboxyl pKs of side chain pKa of a-amino group 2.4 group 9.8 Glycine Aspartate Lysine 2.0 3.9 9.9 2.2 9.1 10.5 A) -2 B) C) D) +2...
14) Which of the following amino acids will by cation-exchange chromatography? following amino acids will be retained longest in the column when sepa Η serne HO-CH-C-COO v. Lysine H3N-CH2CH2CH2CH2CH-co NES NH V. Phenylalanine CH-C–COOH II. Alanine CH3-C-Coo II. Cysteine HS-CH-c-coo® NE 15) What is the product obtained from mild oxidation of the thiol shown below? mild 2R-SH oxidation A) R-S- R B) R-OH C) R-S-S-R DR-OO- RE ) R-S-OH 16) Which bond in the following compound is a peptide bond?...
Match the following amino acids in the described pH to the best description of their net ionic form. Assume the pKa of Lysine's side chain is 10.5 - A. B. C. D. E. Glycine at pH 1. - A. B. C. D. E. Glycine at pH 6. - A. ...
Amino Acids SW C2 9. Which of the following is an L-amino acid? çoo coo coo HẠN |-R HANH, HẠN –H HÀNH R coo B. 10. The following questions refer to the structures A-E below: (a) Which represents the structure of an amino acid at very high pH? (b) Which is a zwitterion? (c) Which represents the structure of an amino acid at very low pH? (d) Which structure/(s) is/are) not possible? R-CH-COOR-CH-COOH R-CH-COOH R-CH-COOR -CH-COO NH2 NH2 *NH NH2...
On paper draw a dipeptide, clearly showing the peptide bond joining the two amino acids together. If the two amino acids are valine and threonine, predict the overall charge of the dipeptide at pH 7. Do not forget to consider the amino (N-terminal) and carboxy (C-terminal) of the dipeptide, as well as the R groups. Select one: a. +2 b. -2 c. 0 d. -1 e. +1
2. Classify the following amino acids as essential or non-essentia ids as essential or non-essential amino acids. b. R c. H d. D e. V f. N g. F h. I i. K j. Q K. A 198
Draw the structure of the following amino acids, at pH 9 i) F ii) P iii) H iv) E v) C vi) G vii) W viii) L
a. Which spot corresponds to the protein with the largest molecular weight? b. When pH = 7, what order would the following peptides elute from a column filled with cation exchange resin? Size exclusion column? Protein 1 – 400 kDa; 10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W, 5%Y Protein 2: 40 kDa 5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R, 5%S, 5%T, 5%V, 5%W, Protein 3 – 175...