Question

1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The images above show a northern blot using a probe to identify the let-7 miRNA (left), and a western blot (right) with a primary antibody to recognize Lin-41, a let-7 target. Assume each lane in both the northern and western blots come from the same sample. Which lane best matches each scenario described listed below? Normally processed let-7 miRNA let-7 miRNA in a Drosha mutant [Choose] [Choose] none of the above lane 5 lane 1 lane 3 lane 4 lane 2 let-7 miRNA in a Dicer mutant let-7 miRNA in an Argonaute mutant [Choose ]

Answer 1

Western blotting is done for protein

mothers blotting is for RNA

1.normally processes rna - lane 4

2. drosha mutant-lane 2
3. dicer mutant- lane 1

arogonaute mutant- lane 5