and plz explain how to prepare and do the protein sequencing
Homework Answers
Isolate rat rung Golgi membranes using sucrose gradient centrifugation of rat lung cell homozinate prepared using grinding with buffer - subcellular fractionation
From the subcellular parts, salting out methods can be used to isolate the proteins by increasing the salt concentration to precipate protein
Once protein is prepared, it can be run on the western blot gel and proteins alone can be purified and sequenced using Sanger sequencjng
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and plz explain how to prepare and do the protein
sequencing
On a piece of paper,...
POST-LAB QUESTIONS 1. Based on the results of the three tests, how do you kno the fruit was DNA and not protein? Explai...
POST-LAB QUESTIONS 1. Based on the results of the three tests, how do you kno the fruit was DNA and not protein? Explain. 2. What happened to the egg white sample, containing mostly protein, when you heated it or oered the pH? Explain what you saw and what you think was happening at the molecular level. 3. Why does the DNA double helix unravel when exposed to high temperatures or acidic conditions? If you tried to run this experiment with...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the...
When a protein is co-translationally translocated into the ER it sometimes receives attachments of eight mannose...
When a protein is co-translationally translocated into the ER it sometimes receives attachments of eight mannose residues, which are added to a core carbohydrate unit consisting of GlcNAc (N-acetylglucosamine). The mannose residues are trimmed down from a unit of eight to a unit of five either in the ER prior to transport to the Golgi, or in the cis-Golgi after transport The removal of three mannose residues can be detected using gel electrophoresis because proteins modified in this way will...
yes a paragraph for both On a separate piece of paper describe (in one paragraph) the...
yes
a paragraph
for
both
On a separate piece of paper describe (in one paragraph) the evidence that the liquid exists in gaseous form at room temperature. Describe two or three ways in which the measurements you made could lead to errors in the results. Explain how each error would affect the answer of molar mass. In other words, would the molar mass go up or down and why?
can someone plz explain all steps fural on how to solve this and why you do...
can someone plz explain all steps fural on how to solve this and
why you do all the steps thx
1.10 uL of a 10uM concentration Cuck- solution mixes with 1-104mL of a 0,002 mm concentration solution of iron(111) cloride concentration = 0,002 nM. caloulate the ending concentration of ar ions in the resulting solution - Answere is 7,27PM
Questions 1-12 Using Key Terms For each pair of terms, explain how the meanings of the...
Questions 1-12
Using Key Terms For each pair of terms, explain how the meanings of the terms differ. 1. producer and consumer 2. lipid and phospholipid Critical Thinking 9. Making Inferences Could life as we know it exist on Earth if air contained only oxygen? Explain. 10. Identifying Relationships Understanding Key Ideas 3. Plants store extra sugar as How might a cave, an ant, and a lake each meet the needs of an organism? a. proteins b, starch. c. nucleic...
Lab #14 Protein Synthesis Introduction Proteins are vital for the survival of an organism. Proteins make...
Lab #14 Protein Synthesis Introduction Proteins are vital for the survival of an organism. Proteins make enzymes and hormones which control reactions that must take place in the cell to survive.Proteins are made of basic units called amino acids. There are a total of 20 amino acids. Different proteins have different number and/or combination of amino acids. The kind of amino acid that is used when producing the protein depends on the 3-base code (codon) read from the RNA molecule...
Betergents h. What chemical would you add to refold this protein? 16. You wish to purify...
Betergents h. What chemical would you add to refold this protein? 16. You wish to purify a novel protein from mammalian cells. You do not know this protein's size or charge, and are thus unsure of which steps to take. Fortunately, an antibody for this protein is available. You have two cell lines: one that expresses the protein, and one • that does not. . ) 4 5 III111 You run both cell lines on an SDS-PAGE (A) followed by...
In laboratory, describe step by step how do you purify protein Trastuzumab or Herceptin by using...
In laboratory, describe step by step how do you purify protein Trastuzumab or Herceptin by using ÄKTA PURE, and chromatographies . You have produced protein TRASTUZUMAB intracellularly in HEK293K. You need to do protein purification now. How would you du it? Describe and motivate
POST-LAB QUESTIONS 1. Based on the results of the three tests, how do you kno the fruit was DNA and not protein? Explain. 2. What happened to the egg white sample, containing mostly protein, when you heated it or oered the pH? Explain what you saw and what you think was happening at the molecular level. 3. Why does the DNA double helix unravel when exposed to high temperatures or acidic conditions? If you tried to run this experiment with...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the...
When a protein is co-translationally translocated into the ER it sometimes receives attachments of eight mannose residues, which are added to a core carbohydrate unit consisting of GlcNAc (N-acetylglucosamine). The mannose residues are trimmed down from a unit of eight to a unit of five either in the ER prior to transport to the Golgi, or in the cis-Golgi after transport The removal of three mannose residues can be detected using gel electrophoresis because proteins modified in this way will...
yes
a paragraph
for
both
On a separate piece of paper describe (in one paragraph) the evidence that the liquid exists in gaseous form at room temperature. Describe two or three ways in which the measurements you made could lead to errors in the results. Explain how each error would affect the answer of molar mass. In other words, would the molar mass go up or down and why?
can someone plz explain all steps fural on how to solve this and
why you do all the steps thx
1.10 uL of a 10uM concentration Cuck- solution mixes with 1-104mL of a 0,002 mm concentration solution of iron(111) cloride concentration = 0,002 nM. caloulate the ending concentration of ar ions in the resulting solution - Answere is 7,27PM
Questions 1-12
Using Key Terms For each pair of terms, explain how the meanings of the terms differ. 1. producer and consumer 2. lipid and phospholipid Critical Thinking 9. Making Inferences Could life as we know it exist on Earth if air contained only oxygen? Explain. 10. Identifying Relationships Understanding Key Ideas 3. Plants store extra sugar as How might a cave, an ant, and a lake each meet the needs of an organism? a. proteins b, starch. c. nucleic...
Lab #14 Protein Synthesis Introduction Proteins are vital for the survival of an organism. Proteins make enzymes and hormones which control reactions that must take place in the cell to survive.Proteins are made of basic units called amino acids. There are a total of 20 amino acids. Different proteins have different number and/or combination of amino acids. The kind of amino acid that is used when producing the protein depends on the 3-base code (codon) read from the RNA molecule...
Betergents h. What chemical would you add to refold this protein? 16. You wish to purify a novel protein from mammalian cells. You do not know this protein's size or charge, and are thus unsure of which steps to take. Fortunately, an antibody for this protein is available. You have two cell lines: one that expresses the protein, and one • that does not. . ) 4 5 III111 You run both cell lines on an SDS-PAGE (A) followed by...
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