Question

In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the localization of each protein (1-4) will be affected by the following mutations in the table below. Protein 3 Protein 1 Where will the protein be localized? Protein 2 Where will the protein be localized? Where will the protein be localized? Protein 4 Where will the protein be localized? Mutation A inactivates the SRP (signal recognition particle) in the cell Mutation B removes the signal sequence from protein 2 Mutation C removes the signal sequence from protein 3 Mutation D prevents the fusion of all vesicles to the cell membrane b. What can you predict about the function of proteins 1-47 Although we do not know specific functions of the protein, decide if each protein will be able to function like a wild-type version of the protein Protein 1 Protein 2 Protein 3 Protein 4 Will this protein function as normal? Will this protein function as normal? Will this protein function as normal? Will this protein function as normal? Mutation A inactivates the SRP (signal recognition particle) in the cell

Answer 1

	A	B	C	D
Mutation A	Cytosol	Cytosol	Nucleus	Membrane
Mutation B	Cytosol	Cytosol	Nucleus	Membrane
Mutation C	Cytosol	Cytosol	Cytosol	Membrane
Mutation D	Cytosol	Cytosol	Nucleus	Cytosol