Question

1. You are interested in the mobility of two different unknown integral plasma membrane proteins—protein X and protein Y—in a cell. In one cell, you fluorescently label all the X proteins. In another cell, you label all the Y proteins. When you perform FRAP analysis on both cells, you find that most of the protein X fluorescence has recovered within 3 minutes, but recovery of the protein Y fluorescence is significantly slower. Even after 10 minutes, only 50% of the protein Y fluorescence has recovered. Describe the FRAP experimental protocol, explain what the results tell you about mobility of the X and Y proteins, and propose a possible explanation for why the mobilities of the two proteins differ.

Answer 1

Answer:- i The FRAP analysis į mainly used to track the protuns tagged with GfP bresion proteins. when these x and y protuus are tagged with GfP proteins and when they are Enited by a Speutic wavelength of light, the protein will theoresie. 1 x protein has recovered fast and y protein was recovered at a shower rate this shows that the y protein got bound with a receptor which might have been a static receptor protein and hence the recovery rate was slower in comparison with the x profun ill, The difference in mobility of protun Explains the rate of binding of each protein to an immobile 8 static receptor then the rate of fluorescence and recovery will be retarded w ith please Rate my Ansuar - =