Question

16-18 DNA Fingerprinting be assembled in order during the amplification process? Which ingredier process? Which ingredient guides the placement of the structural componen correct positions? Alication process? Which ingredients begin the at of the structural components into their 8. The PCR process occurs at three different temperatures. What happens at ... a 95°? b. 37°-60°? 6 72°? 9. What is meant by "annealing when it is applied to the PCR process? 10. The thermocycler repeats the temperature cycle many times. What happens to the DNA during each of these cycles? 11. In electrophoresis, why is it important that the DNA samples be placed in the gel at the edge closer to the negative electrode? 12. In electrophoresis, some bands move farther through the gel than others. What determines the distance the DNA in the bands will move during the electrophoresis running time 382

Answer 1

97	melting of DNA
37- 60	Annealing
72	Extending

The two DNA are stranded at the first stage, high temperature is due to denaturing the DNA.

Attachment of the primer at the separated strand is done at the annealing step.

The extension is done at the third stage, and temperature is around 72.

Double stranded DNA. Denaturing Extending of Innealing stage v 50-56°c 72°C roumerare chain reaction Scanned fith Canscanner

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