NA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the...
NA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed.
1)Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells.
2)An electric current is passed through the gel.
3) DNA fragments are treated with a dye.
4)A restriction endonuclease is added to the DNA.
5)Using micropipettes, the DNA samples are added to the wells.
6)DNA fingerprint is produced.
7)DNA fragments are produced.
The order in which a DNA fingerprint is produced using gel electrophoresis is:___,____,___,____,____,____,____,____
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NA fingerprinting uses a process called gel electrophoresis to
separate the fragments of DNA. Once the...
Stuck answering the rest of these 3. Application of DNA gel electrophoresis. DNA gel electrophoresis is...
Stuck answering the rest of
these
3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
16-18 DNA Fingerprinting be assembled in order during the amplification process? Which ingredier process? Which ingredient...
16-18 DNA Fingerprinting be assembled in order during the amplification process? Which ingredier process? Which ingredient guides the placement of the structural componen correct positions? Alication process? Which ingredients begin the at of the structural components into their 8. The PCR process occurs at three different temperatures. What happens at ... a 95°? b. 37°-60°? 6 72°? 9. What is meant by "annealing when it is applied to the PCR process? 10. The thermocycler repeats the temperature cycle many times....
One strand of a DNA sequences is given below. Find the EcoRI sites and indicate the...
One strand of a DNA sequences is given below. Find the
EcoRI sites and indicate the cutting site with an arrow. Count the
number of bases in each fragment.
CP22: vne strand of a DNA sequence is given below. Find the EcoRI sites and indicate the cutting site with an arrow. Count the number of bases in each fragment. Restriction digest A: ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC Number of bases in each fragment: Now compare the same region of DNA from another individual. Where...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
After PCR is performed the products are run out on an agarose gel. In the figure...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
L= No restriction B=Bam HI E=EcoRI H=Hind III Band 1 27mm 31mm 29mm 29mm Band 2...
L= No restriction
B=Bam HI
E=EcoRI
H=Hind III
Band 1
27mm
31mm
29mm
29mm
Band 2
NA
34mm
41mm
37mm
Band 3
NA
41mm
46mm
43mm
Band 4
NA
43mm
49mm
52mm
Band 5
NA
46mm
57mm
71mm
Band 6
NA
NA
NA
76mm
Above is the actual measurements for the distance in mm. Please
plug this in with the existing chart located above
Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...
Hi can someone help me understand part C and why the drawn in red lines are...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
Stuck answering the rest of
these
3. Application of DNA gel electrophoresis. DNA gel electrophoresis is commonly used in determining familial relationships among individuals, for ex; to establish paternity of a child. This technique is called DNA fingerprinting. In this technique the DNA of parents and children is roughly chopped up into pieces and resolved on an agarose gel. The DNA ill resolve according to their sizes and create a pattern or a "fingerprint". The fingerprint of the child is...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
16-18 DNA Fingerprinting be assembled in order during the amplification process? Which ingredier process? Which ingredient guides the placement of the structural componen correct positions? Alication process? Which ingredients begin the at of the structural components into their 8. The PCR process occurs at three different temperatures. What happens at ... a 95°? b. 37°-60°? 6 72°? 9. What is meant by "annealing when it is applied to the PCR process? 10. The thermocycler repeats the temperature cycle many times....
One strand of a DNA sequences is given below. Find the
EcoRI sites and indicate the cutting site with an arrow. Count the
number of bases in each fragment.
CP22: vne strand of a DNA sequence is given below. Find the EcoRI sites and indicate the cutting site with an arrow. Count the number of bases in each fragment. Restriction digest A: ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC Number of bases in each fragment: Now compare the same region of DNA from another individual. Where...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
L= No restriction
B=Bam HI
E=EcoRI
H=Hind III
Band 1
27mm
31mm
29mm
29mm
Band 2
NA
34mm
41mm
37mm
Band 3
NA
41mm
46mm
43mm
Band 4
NA
43mm
49mm
52mm
Band 5
NA
46mm
57mm
71mm
Band 6
NA
NA
NA
76mm
Above is the actual measurements for the distance in mm. Please
plug this in with the existing chart located above
Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
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